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验证数据展示

  • WB analysis of Jurkat using 17427-1-AP

    WB analysis of Jurkat using 17427-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17427-1-AP (NARG1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 17427-1-AP

    IF Staining of HEK-293 using 17427-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NARG1L antibody (17427-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NARG1L Polyclonal antibody

NARG1L Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17427-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

NMDA receptor-regulated 1-like protein, NAT2, NARG1-like protein, NARG1 like protein, N-alpha-acetyltransferase 16, NatA auxiliary subunit

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17427-1-AP targets NARG1L in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NARG1L fusion protein Ag11492 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NMDA receptor regulated 1-like
别名 NMDA receptor-regulated 1-like protein, NAT2, NARG1-like protein, NARG1 like protein, N-alpha-acetyltransferase 16, NatA auxiliary subunit
计算分子量 864 aa, 101 kDa
观测分子量 97-100 kDa
GenBank蛋白编号BC028112
基因名称 NARG1L
Gene ID (NCBI) 79612
RRIDAB_1939557
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6N069
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NMDA receptor-regulated 1-like protein (NARG1L, also known as NAA16), is the auxillary subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity.

实验方案

Product Specific Protocols
WB protocol for NARG1L antibody 17427-1-APDownload protocol
IF protocol for NARG1L antibody 17427-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols