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NAP1L4 Polyclonal antibody

NAP1L4 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16018-1-AP

Print datasheet

Synonyms

hNAP2, NAP 2, NAP1L4, NAP2, NAP2L, Nucleosome assembly protein 2



经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue, mouse testis tissue, HEK-293 cells, A431 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16018-1-AP targets NAP1L4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen NAP1L4 fusion protein Ag8827 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nucleosome assembly protein 1-like 4
Synonyms hNAP2, NAP 2, NAP1L4, NAP2, NAP2L, Nucleosome assembly protein 2
Calculated Molecular Weight 375 aa, 43 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC022090
Gene Symbol NAP1L4
Gene ID (NCBI) 4676
RRIDAB_2150711
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99733
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NAP1L4 antibody 16018-1-APDownload protocol
IHC protocol for NAP1L4 antibody 16018-1-APDownload protocol
IP protocol for NAP1L4 antibody 16018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Biol

Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine.

Authors - Chen Chen
humanWB

Mol Cell Proteomics

A novel immuno-competitive capture mass spectrometry strategy for protein-protein interaction profiling reveals that LATS kinases regulate HCV replication through NS5A phosphorylation.

Authors - Hélène Meistermann
mouseIF,WB

J Virol

NAP1L1 and NAP1L4 binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein is bivalent and requires phosphorylation.

Authors - Francisco Dominguez
humanWB,IHC

J Dermatol Sci

Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells.

Authors - Satoru Mizuhashi
  • KD Validated
humanWB

Nat Struct Mol Biol

Mechanism of client selection by the protein quality-control factor UBE2O

Authors - Matthew C J Yip
humanWB

iScience

Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges

Authors - Tamami Miyagi