验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, HT-1080 cells, A431 cells, HEK-293 cells, mouse thymus tissue, HL-60 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, NIH/3T3 cells |
| Positive FC (Intra) detected in | NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy NAP1L1 Ready-To-Use IHC Kit. NAP1L1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14898-1-AP targets NAP1L1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | NAP1L1 fusion protein Ag6690 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nucleosome assembly protein 1-like 1 |
| 别名 | Nucleosome assembly protein 1-like 1, NRP, NAP-1-related protein, NAP1L, NAP1 |
| 计算分子量 | 45 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | BC002387 |
| 基因名称 | NAP1L1 |
| Gene ID (NCBI) | 4673 |
| RRID | AB_2150696 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55209 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NAP1L1 antibody 14898-1-AP | Download protocol |
| IHC protocol for NAP1L1 antibody 14898-1-AP | Download protocol |
| IF protocol for NAP1L1 antibody 14898-1-AP | Download protocol |
| IP protocol for NAP1L1 antibody 14898-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hum Reprod Whole exome sequencing in unexplained recurrent miscarriage families identified novel pathogenic genetic causes of euploid miscarriage | ||
Front Cell Dev Biol NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma.
| ||
Oncotarget Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer. | ||
J Virol NAP1L1 and NAP1L4 binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein is bivalent and requires phosphorylation. | ||
Cancer Cell Int NAP1L1 interacts with hepatoma-derived growth factor to recruit c-Jun inducing breast cancer growth.
| ||
J Dermatol Sci Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells. |
