验证数据展示

WB analysis using 14898-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 14898-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).
Ready-to-use IHC kits available with NAP1L1 antibody included
WB analysis of HeLa using 14898-1-AP
WB result of NAP1L1 antibody (14898-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAP1L1 transfected HeLa cells.
IHC staining of mouse testis using 14898-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14898-1-AP (NAP1L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with NAP1L1 antibody included
IHC staining of rat spleen using 14898-1-AP
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 14898-1-AP (NAP1L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with NAP1L1 antibody included
FC experiment of NIH/3T3 using 14898-1-AP
1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug NAP1L1 Polyclonal antibody (14898-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IP experiment of HEK-293 using 14898-1-AP
IP result of anti-NAP1L1 (IP:14898-1-AP, 4ug; Detection:14898-1-AP 1:5000) with HEK-293 cells lysate 1440 ug.
IF Staining of HeLa using 14898-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAP1L1 antibody (14898-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
IF Staining of NIH/3T3 using 14898-1-AP
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using NAP1L1 antibody (14898-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO验证

NAP1L1 Polyclonal antibody

NAP1L1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14898-1-AP

产品说明书

引用文献(11)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

别名

NAP1, NAP1L, NAP-1-related protein, NRP, Nucleosome assembly protein 1-like 1

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (11)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HL-60 cells, HeLa cells, HEK-293 cells, HT-1080 cells, A431 cells, mouse thymus tissue, Jurkat cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	mouse testis tissue, human heart tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human spleen tissue, human lung tissue, human ovary tissue, rat spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, NIH/3T3 cells
Positive FC (Intra) detected in	NIH/3T3 cells

Planning an IHC experiment? We recommend our IHCeasy NAP1L1 Ready-To-Use IHC Kit. NAP1L1 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 4 publications below
WB	See 8 publications below
IHC	See 1 publications below
IF	See 5 publications below

产品信息

14898-1-AP targets NAP1L1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse
免疫原	NAP1L1 fusion protein Ag6690 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	nucleosome assembly protein 1-like 1
别名	NAP1, NAP1L, NAP-1-related protein, NRP, Nucleosome assembly protein 1-like 1
计算分子量	45 kDa
观测分子量	55-60 kDa
GenBank蛋白编号	BC002387
基因名称	NAP1L1
Gene ID (NCBI)	4673
RRID	AB_2150696
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P55209
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

实验方案

Product Specific Protocols
WB protocol for NAP1L1 antibody 14898-1-AP	Download protocol
IHC protocol for NAP1L1 antibody 14898-1-AP	Download protocol
IF protocol for NAP1L1 antibody 14898-1-AP	Download protocol
IP protocol for NAP1L1 antibody 14898-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IF	Hum Reprod Whole exome sequencing in unexplained recurrent miscarriage families identified novel pathogenic genetic causes of euploid miscarriage Authors - Xiyao Wang View Article
human	WB	Front Cell Dev Biol NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma. Authors - Ye-Wei Zhang KD Validated View Article
human	WB	Oncotarget Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer. Authors - Liyuan Zhang View Article
mouse	IF,WB	J Virol NAP1L1 and NAP1L4 binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein is bivalent and requires phosphorylation. Authors - Francisco Dominguez View Article
human	WB,IHC,IF	Cancer Cell Int NAP1L1 interacts with hepatoma-derived growth factor to recruit c-Jun inducing breast cancer growth. Authors - Shu Liu KD Validated View Article
human	WB	J Dermatol Sci Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells. Authors - Satoru Mizuhashi View Article

验证数据展示

WB analysis using 14898-1-AP

IHC staining of human testis using 14898-1-AP

WB analysis of HeLa using 14898-1-AP

IHC staining of mouse testis using 14898-1-AP

IHC staining of rat spleen using 14898-1-AP

FC experiment of NIH/3T3 using 14898-1-AP

IP experiment of HEK-293 using 14898-1-AP

IF Staining of HeLa using 14898-1-AP

IF Staining of NIH/3T3 using 14898-1-AP

NAP1L1 Polyclonal antibody

NAP1L1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

实验方案

发表文章

Whole exome sequencing in unexplained recurrent miscarriage families identified novel pathogenic genetic causes of euploid miscarriage

NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma.

Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.

NAP1L1 and NAP1L4 binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein is bivalent and requires phosphorylation.

NAP1L1 interacts with hepatoma-derived growth factor to recruit c-Jun inducing breast cancer growth.

Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells.