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验证数据展示

  • WB analysis using 14898-1-AP

    WB analysis using 14898-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 14898-1-AP

    IHC staining of human testis using 14898-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with NAP1L1 antibody included
  • WB analysis of HeLa using 14898-1-AP

    WB analysis of HeLa using 14898-1-AP

    WB result of NAP1L1 antibody (14898-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAP1L1 transfected HeLa cells.

  • IHC staining of mouse testis using 14898-1-AP

    IHC staining of mouse testis using 14898-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14898-1-AP (NAP1L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NAP1L1 antibody included
  • IHC staining of rat spleen using 14898-1-AP

    IHC staining of rat spleen using 14898-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 14898-1-AP (NAP1L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NAP1L1 antibody included
  • FC experiment of NIH/3T3 using 14898-1-AP

    FC experiment of NIH/3T3 using 14898-1-AP

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug NAP1L1 Polyclonal antibody (14898-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IP experiment of HEK-293 using 14898-1-AP

    IP experiment of HEK-293 using 14898-1-AP

    IP result of anti-NAP1L1 (IP:14898-1-AP, 4ug; Detection:14898-1-AP 1:5000) with HEK-293 cells lysate 1440 ug.

  • IF Staining of HeLa using 14898-1-AP

    IF Staining of HeLa using 14898-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAP1L1 antibody (14898-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • IF Staining of NIH/3T3 using 14898-1-AP

    IF Staining of NIH/3T3 using 14898-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using NAP1L1 antibody (14898-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Featured Product
  • KD/KO Validated

NAP1L1 Polyclonal antibody

NAP1L1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 14898-1-AP

产品说明书

引用文献(17)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

别名

NAP1, NAP1L, NAP-1-related protein, NRP, Nucleosome assembly protein 1-like 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells, HEK-293 cells, HT-1080 cells, A431 cells, mouse thymus tissue, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse testis tissue, human heart tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human spleen tissue, human lung tissue, human ovary tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, NIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy NAP1L1 Ready-To-Use IHC Kit. NAP1L1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14898-1-AP targets NAP1L1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag6690

Product name: Recombinant human NAP1L1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-391 aa of BC002387

Sequence: MADIDNKEQSELDQDLDDVEEVEEEETGEETKLKARQLTVQMMQNPQILAALQERLDGLVETPTGYIESLPRVVKRRVNALKNLQVKCAQIEAKFYEEVHDLERKYAVLYQPLFDKRFEIINAIYEPTEEECEWKPDEEDEISEELKEKAKIEDEKKDEEKEDPKGIPEFWLTVFKNVDLLSDMVQEHDEPILKHLKDIKVKFSDAGQPMSFVLEFHFEPNEYFTNEVLTKTYRMRSEPDDSDPFSFDGPEIMGCTGCQIDWKKGKNVTLKTIKKKQKHKGRGTVRTVTKTVSNDSFFNFFAPPEVPESGDLDDDAEAILAADFEIGHFLRERIIPRSVLYFTGEAIEDDDDDYDEEGEEADEEGEEEGDEENDPDYDPKKDQNPAECKQQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleosome assembly protein 1-like 1
别名 NAP1, NAP1L, NAP-1-related protein, NRP, Nucleosome assembly protein 1-like 1
计算分子量 45 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC002387
基因名称 NAP1L1
Gene ID (NCBI) 4673
RRIDAB_2150696
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55209
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for NAP1L1 antibody 14898-1-APDownload protocol
IF protocol for NAP1L1 antibody 14898-1-APDownload protocol
IHC protocol for NAP1L1 antibody 14898-1-APDownload protocol
IP protocol for NAP1L1 antibody 14898-1-APDownload protocol
WB protocol for NAP1L1 antibody 14898-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Signal Transduct Target Ther

Nucleosome assembly protein-like 1 degradation-dependent novel cardioprotection mechanism of Wnt2 against ischemia‒reperfusion injury

Authors - Ying Wang
View Article
humanWB

Mol Cancer

NAP1L1 degradation by FBXW7 reduces the deubiquitination of HDGF-p62 signaling to stimulate autophagy and induce primary cisplatin chemosensitivity in nasopharyngeal carcinoma

Authors - Bin Gong
  • KD Validated
View Article

Autophagy

Restricting intracellular Salmonella proliferation by coordinating p-TBK1 mediated mitophagy and xenophagy

Authors - Jun Li
View Article
human

Protein Cell

CRISPR-assisted transcription activation by phase separation proteins

Authors - Jiaqi Liu
View Article
humanWB

J Adv Res

Long noncoding RNA AC007637.1 inhibits tumorigenesis by regulating the Trim25/PCNA axis in colorectal cancer.

Authors - Xue Wang
View Article
humanWB

Nat Struct Mol Biol

Mechanism of client selection by the protein quality-control factor UBE2O

Authors - Matthew C J Yip
View Article
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