验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, human brain tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 2 publications below |
产品信息
15163-1-AP targets NANS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag7271 Product name: Recombinant human NANS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-359 aa of BC000008 Sequence: MPLELELCPGRWVGGQHPCFIIAEIGQNHQGDLDVAKRMIRMAKECGADCAKFQKSELEFKFNRKALERPYTSKHSWGKTYGEHKRHLEFSHDQYRELQRYAEEVGIFFTASGMDEMAVEFLHELNVPFFKVGSGDTNNFPYLEKTAKKGRPMVISSGMQSMDTMKQVYQIVKPLNPNFCFLQCTSAYPLQPEDVNLRVISEYQKLFPDIPIGYSGHETGIAISVAAVALGAKVLERHITLDKTWKGSDHSASLEPGELAELVRSVRLVERALGSPTKQLLPCEMACNEKLGKSVVAKVKIPEGTILTMDMLTVKVGEPKGYPPEDIFNLVGKKVLVTVEEDDTIMEELVDNHGKKIKS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | N-acetylneuraminic acid synthase |
| 别名 | N-acetylneuraminic acid phosphate synthase, N-acetylneuraminate-9-phosphate synthase, N-acetylneuraminate synthase, N acetylneuraminate synthase, EC:2.5.1.57 |
| 计算分子量 | 40 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC000008 |
| 基因名称 | NANS |
| Gene ID (NCBI) | 54187 |
| RRID | AB_2150690 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NR45 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The sialic acids are a family of 9-carbon 2-keto-3-deoxy sugars. They are frequently the terminal sugars on secreted and cell surface glycoproteins and glycolipids. Sialic acids participate in many important biologic recognition events. NANS can also use N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NANS antibody 15163-1-AP | Download protocol |
| IP protocol for NANS antibody 15163-1-AP | Download protocol |
| WB protocol for NANS antibody 15163-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Gastroenterol Hepatol The Role of the Hexosamine-Sialic Acid Metabolic Pathway Mediated by GFPT1/NANS in c-Myc-Driven Hepatocellular Carcinoma | ||
Exp Cell Res Reanalysis of single-cell data reveals macrophage subsets associated with the immunotherapy response and prognosis of patients with endometrial cancer | ||
Front Immunol Identification and verification of the optimal feature genes of ferroptosis in thyroid-associated orbitopathy |
