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验证数据展示

  • WB analysis using 12519-1-AP

    WB analysis using 12519-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12519-1-AP (NANP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 12519-1-AP

    IHC staining of human ovary cancer using 12519-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12519-1-AP (NANP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 12519-1-AP

    IF Staining of A431 using 12519-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using NANP antibody (12519-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NANP Polyclonal antibody

NANP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12519-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Neu5Ac-9-Pase, N-acylneuraminate-9-phosphatase, HDHD4, Haloacid dehalogenase-like hydrolase domain-containing protein 4, EC:3.1.3.29

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, Neuro-2a cells, mouse brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12519-1-AP targets NANP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3212

Product name: Recombinant human NANP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-248 aa of BC022552

Sequence: MGLSRVRAVFFDLDNTLIDTAGASRRGMLEVIKLLQSKYHYKEEAEIICDKVQVKLSKECFHPYNTCITDLRTSHWEEAIQETKGGAANRKLAEECYFLWKSTRLQHMTLAEDVKAMLTELRKEVRLLLLTNGDRQTQREKIEACACQSYFDAVVVGGEQREEKPAPSIFYYCCNLLGVQPGDCVMVGDTLETDIQGGLNAGLKATVWINKNGIVPLKSSPVPHYMVSSVLELPALLQSIDCKVSMST

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-acetylneuraminic acid phosphatase
别名 Neu5Ac-9-Pase, N-acylneuraminate-9-phosphatase, HDHD4, Haloacid dehalogenase-like hydrolase domain-containing protein 4, EC:3.1.3.29
计算分子量 248 aa, 28 kDa
观测分子量 29 kDa
GenBank蛋白编号BC022552
基因名称 NANP
Gene ID (NCBI) 140838
RRIDAB_3085396
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TBE9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NANP, also known as HDHD4, C20orf147, is a member of the haloacid dehalogenase family of proteins. NANP enables N-acylneuraminate-9-phosphatase activity and is involved in N-acetylneuraminate biosynthetic process.

实验方案

Product Specific Protocols
IF protocol for NANP antibody 12519-1-APDownload protocol
IHC protocol for NANP antibody 12519-1-APDownload protocol
WB protocol for NANP antibody 12519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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