• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NAGS Polyclonal antibody

NAGS Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 21566-1-AP

Print datasheet

Synonyms

AGAS, Amino acid acetyltransferase, ARGA, N acetylglutamate synthase, NAGS

验证数据展示

  • WB analysis of HepG2 using 21566-1-AP

    WB analysis of HepG2 using 21566-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 21566-1-AP

    IHC staining of human liver using 21566-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21566-1-AP (NAGS antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 21566-1-AP

    IHC staining of human liver using 21566-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21566-1-AP (NAGS antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 21566-1-AP

    IF Staining of HepG2 using 21566-1-AP

    Immunofluorescent analysis of HepG2 cells, using NAGS antibody 21566-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, L02 cells
Positive IHC detected inhuman liver tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21566-1-AP targets NAGS in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen NAGS fusion protein Ag16073 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-acetylglutamate synthase
Synonyms AGAS, Amino acid acetyltransferase, ARGA, N acetylglutamate synthase, NAGS
Calculated Molecular Weight 534 aa, 58 kDa
Observed Molecular Weight 58-66 kDa
GenBank Accession NumberBC111713
Gene Symbol NAGS
Gene ID (NCBI) 162417
RRIDAB_10733886
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N159
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

N-acetylglutamate synthase, also known as NAGS, is a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAGS is the key enzyme for regulating the hepatic urea cycle and is also highly expressed in the kidney and gut. Deficiency of NAGS leads to hyperammonemia due to decreased activity of CPSI deprived of its cofactor NAG(PMID:12459178,12754705).

实验方案

Product Specific Protocols
WB protocol for NAGS antibody 21566-1-APDownload protocol
IHC protocol for NAGS antibody 21566-1-APDownload protocol
IF protocol for NAGS antibody 21566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Metab

SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.

Authors - Wenxiang Zhang
View Article