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验证数据展示

  • WB analysis using 21566-1-AP

    WB analysis using 21566-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 21566-1-AP

    IHC staining of human liver using 21566-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21566-1-AP (NAGS antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 21566-1-AP

    IHC staining of human liver using 21566-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21566-1-AP (NAGS antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 21566-1-AP

    IF Staining of HepG2 using 21566-1-AP

    Immunofluorescent analysis of HepG2 cells, using NAGS antibody 21566-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • WB analysis of HepG2 using 21566-1-AP

    WB analysis of HepG2 using 21566-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

NAGS Polyclonal antibody

NAGS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21566-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

AGAS, Amino acid acetyltransferase, ARGA, N acetylglutamate synthase, NAGS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, Jurkat cells, HepG2 cells, L02 cells, rat liver tissue
Positive IHC detected inhuman liver tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21566-1-AP targets NAGS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 NAGS fusion protein Ag16073 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-acetylglutamate synthase
别名 AGAS, Amino acid acetyltransferase, ARGA, N acetylglutamate synthase, NAGS
计算分子量 534 aa, 58 kDa
观测分子量 58-66 kDa
GenBank蛋白编号BC111713
基因名称 NAGS
Gene ID (NCBI) 162417
RRIDAB_10733886
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N159
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

N-acetylglutamate synthase, also known as NAGS, is a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAGS is the key enzyme for regulating the hepatic urea cycle and is also highly expressed in the kidney and gut. Deficiency of NAGS leads to hyperammonemia due to decreased activity of CPSI deprived of its cofactor NAG(PMID:12459178,12754705).

实验方案

Product Specific Protocols
WB protocol for NAGS antibody 21566-1-APDownload protocol
IHC protocol for NAGS antibody 21566-1-APDownload protocol
IF protocol for NAGS antibody 21566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Metab

SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.

Authors - Wenxiang Zhang
View Article
human,mouseWB,IHC

Heliyon

Machine learning deciphers the significance of mitochondrial regulators on the diagnosis and subtype classification in non-alcoholic fatty liver disease

Authors - Bingyu Wang
View Article