验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, U2OS cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
15096-1-AP targets NAGA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | NAGA fusion protein Ag7172 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | N-acetylgalactosaminidase, alpha- |
| 别名 | GALB, EC:3.2.1.49, Alpha-N-acetylgalactosaminidase, Alpha-galactosidase B, Alpha galactosidase B |
| 计算分子量 | 47 kDa |
| 观测分子量 | 48 kDa, 60-70 kDa |
| GenBank蛋白编号 | BC000095 |
| 基因名称 | NAGA |
| Gene ID (NCBI) | 4668 |
| RRID | AB_2282779 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P17050 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NAGA belongs to the glycosyl hydrolase 27 family. It removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. It is required for the breakdown of glycolipids. Biosynthetic studies performed with cultured fibroblasts indicated that the human enzyme was synthesized as a 65 kDa glycosylated precursor which was processed to a mature 48-kDa lysosomal form; both the precursor and mature forms had high mannose type oligosaccharide chains, but only the precursor's mannose residues were phosphorylated. 90-117 kDa is a homodimer of NAGA.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NAGA antibody 15096-1-AP | Download protocol |
| IHC protocol for NAGA antibody 15096-1-AP | Download protocol |
| IF protocol for NAGA antibody 15096-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |