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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
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Compatible Species
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验证数据展示

  • WB analysis using 84996-1-RR (same clone as 84996-1-PBS)

    WB analysis using 84996-1-RR (same clone as 84996-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84996-1-RR (NADK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84996-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84996-1-RR

    Affinity and Kinetic Characterization of 84996-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84996-1-RR against Human NADK were performed. The affinity constant is 2.25 nM.

NADK Recombinant monoclonal antibody, PBS Only

NADK Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 84996-1-PBS

产品说明书

CloneNo. 242502B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

242502B7, EC:2.7.1.23, NAD kinase, Poly(P)/ATP NAD kinase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84996-1-PBS targets NADK in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag7906

Product name: Recombinant human NADK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 98-446 aa of BC001709

Sequence: LTWNKSPKSVLVIKKMRDASLLQPFKELCTHLMEENMIVYVEKKVLEDPAIASDESFGAVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLGFLTPFSFENFQSQVTQVIEGNAAVVLRSRLKVRVVKELRGKKTAVHNGLGEKGSQAAGLDMDVGKQAMQYQVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAAAAGASMIHPNVPAIMITPICPHSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRHGDSISITTSCYPLPSICVRDPVSDWFESLAQCLHWNVRKKQAHFEEEEEEEEEG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NAD kinase
别名 242502B7, EC:2.7.1.23, NAD kinase, Poly(P)/ATP NAD kinase
计算分子量 49 kDa
观测分子量 49-52 kDa
GenBank蛋白编号BC001709
基因名称 NADK
Gene ID (NCBI) 65220
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95544
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).

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