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NADK Polyclonal antibody

NADK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 29290-1-AP

Print datasheet

Synonyms

dJ283E3.1, FLJ13052, FLJ37724, NAD kinase, NADK, Poly(P)/ATP NAD kinase

验证数据展示

  • WB analysis using 29290-1-AP

    WB analysis using 29290-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29290-1-AP (NADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, Hela cells, Neuro-2a cells, NIH/3T3 cells, rat kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29290-1-AP targets NADK in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen NADK fusion protein Ag30982 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NAD kinase
Synonyms dJ283E3.1, FLJ13052, FLJ37724, NAD kinase, NADK, Poly(P)/ATP NAD kinase
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC001709
Gene Symbol NADK
Gene ID (NCBI) 65220
RRIDAB_2918272
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95544
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).

实验方案

Product Specific Protocols
WB protocol for NADK antibody 29290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols