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验证数据展示

  • WB analysis using 29290-1-AP

    WB analysis using 29290-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29290-1-AP (NADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 29290-1-AP

    WB analysis of HEK-293 using 29290-1-AP

    WB result of NADK antibody (29290-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-NADK transfected HEK-293 cells.

  • IHC staining of human stomach cancer using 29290-1-AP

    IHC staining of human stomach cancer using 29290-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29290-1-AP (NADK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 29290-1-AP

    IHC staining of human ovary cancer using 29290-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29290-1-AP (NADK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

NADK Polyclonal antibody

NADK Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29290-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

EC:2.7.1.23, NAD kinase, Poly(P)/ATP NAD kinase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Hela cells, Neuro-2a cells, NIH/3T3 cells, rat kidney tissue
Positive IHC detected inhuman ovary cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29290-1-AP targets NADK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30982

Product name: Recombinant human NADK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 238-446 aa of BC001709

Sequence: SRLKVRVVKELRGKKTAVHNGLGEKGSQAAGLDMDVGKQAMQYQVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAAAAGASMIHPNVPAIMITPICPHSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRHGDSISITTSCYPLPSICVRDPVSDWFESLAQCLHWNVRKKQAHFEEEEEEEEEG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NAD kinase
别名 EC:2.7.1.23, NAD kinase, Poly(P)/ATP NAD kinase
计算分子量 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC001709
基因名称 NADK
Gene ID (NCBI) 65220
RRIDAB_2918272
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95544
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).

实验方案

Product Specific Protocols
WB protocol for NADK antibody 29290-1-APDownload protocol
IHC protocol for NADK antibody 29290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols