验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, L02 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16576-1-AP targets NAAA in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human |
免疫原 | NAAA fusion protein Ag9861 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | N-acylethanolamine acid amidase |
别名 | ASAH-like protein, ASAHL, ASAH like protein, Acylsphingosine deacylase NAAA, Acid ceramidase-like protein |
计算分子量 | 359 aa, 40 kDa |
观测分子量 | 40 kDa |
GenBank蛋白编号 | BC011854 |
基因名称 | NAAA |
Gene ID (NCBI) | 27163 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q02083 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NAAA (N-acylethanolamine acid amidase), also named ASAHL and PLT, is a novel lysosomal hydrolase structurally and functionally similar to acid ceramidase and is mainly involved in the hydrolysis of fatty amides. NAAA is mainly responsible for the degradation of bioactive fatty amides, such as palmitoylethanolamide (PEA), and is involved in lipid metabolism and fatty acid metabolism processes (PMID: 32191459; PMID: 17712833).
实验方案
Product Specific Protocols | |
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WB protocol for NAAA antibody 16576-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |