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验证数据展示

  • WB analysis using 16698-1-AP (same clone as 16698-1-PBS)

    WB analysis using 16698-1-AP (same clone as 16698-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16698-1-AP (NAT11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16698-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 16698-1-AP (same clone as 16698-1-PBS)

    IHC staining of human ovarian cancer using 16698-1-AP (same clone as 16698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16698-1-AP (NAA40 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16698-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 16698-1-AP (same clone as 16698-1-PBS)

    IF Staining of U2OS using 16698-1-AP (same clone as 16698-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NAA40 antibody (16698-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16698-1-PBS in a different storage buffer formulation.

NAA40 Polyclonal antibody, PBS Only

NAA40 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16698-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

NAT11, N-alpha-acetyltransferase 40, N-acetyltransferase 11, N alpha acetyltransferase 40, NatD catalytic subunit, N acetyltransferase 11

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16698-1-PBS targets NAA40 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10092

Product name: Recombinant human NAT11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-237 aa of BC052298

Sequence: MGRKSSKAKEKKQKRLEERAAMDAVCAKVDAANRLGDPLEAFPVFKKYDRNGLNVSIECKRVSGLEPATVDWAFDLTKTNMQTMYEQSEWGWKDREKREEMTDDRAWYLIAWENSSVPVAFSHFRFDVECGDEVLYCYEVQLESKVRRKGLGKFLIQILQLMANSTQMKKVMLTVFKHNHGAYQFFREALQFEIDDSSPSMSGCCGEDCSYEILSRRTKFGDSHHSHAGGHCGGCCH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-acetyltransferase 11 (GCN5-related, putative)
别名 NAT11, N-alpha-acetyltransferase 40, N-acetyltransferase 11, N alpha acetyltransferase 40, NatD catalytic subunit, N acetyltransferase 11
计算分子量 237 aa, 27 kDa
观测分子量27 kDa
GenBank蛋白编号BC052298
基因名称 NAA40
Gene ID (NCBI) 79829
RRIDAB_3085505
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86UY6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

N-alpha-acetyltransferase 40 (NAA40, also known as NatD, Nat4, or Patt1) is a highly specific epigenetic enzyme catalyzing the transfer of an acetyl moiety at the N-terminal end of histones H4 and H2A (PMID: 26666750). NAA40 is significantly upregulated in primary CRC tissues and promotes CRC cell growth both in vitro and in xenograft tumor models (PMID: 30858358). Western blot analysis detected NAA40 at an apparent molecular mass of 27 kDa.

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