N-cadherin Polyclonal antibody

N-cadherin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30803-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Rat and More (1)

应用

WB, IHC, IF/ICC, ELISA

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHeLa cells, rat brain tissue, PC-3 cells, C2C12 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy N-cadherin Ready-To-Use IHC Kit. N-cadherin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

30803-1-AP targets N-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, Mouse, Rat
文献引用反应性human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cadherin 2, type 1, N-cadherin (neuronal)
别名 Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin
计算分子量100 kDa
观测分子量130 kDa
GenBank蛋白编号NM_001792.4
基因名称 N-cadherin
Gene ID (NCBI) 1000
RRIDAB_3669759
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19022-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619).

实验方案

Product Specific Protocols
WB protocol for N-cadherin antibody 30803-1-APDownload protocol
IHC protocol for N-cadherin antibody 30803-1-APDownload protocol
IF protocol for N-cadherin antibody 30803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Genet

Mechanistic study of Liquiritigenin inhibiting bladder cancer cell proliferation and migration by regulating STING1

Authors - Wuheng Li
Loading...