Product Information

KE30009 is a vitro competitive binding enzyme-linked immunosorbent assay (Competitive ELISA). The testosterone ELISA kit is to be used to detect and quantify protein levels of vitro testosterone. A 96-well plate has been precoated with Testosterone antigen. Samples and the antibody-HRP conjugate are added to the wells, where testosterone in the sample competes with the testosterone antigen immobilized on the microplate for binding with the antibody-HRP. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Testosterone in the sample and the intensity is measured at 450 nm with the correction wavelength set at 630 nm.