• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human skeletal muscle using 66205-1-Ig (same clone as 66205-1-PBS)

    WB analysis of human skeletal muscle using 66205-1-Ig (same clone as 66205-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

  • IHC staining of rat heart using 66205-1-Ig (same clone as 66205-1-PBS)

    IHC staining of rat heart using 66205-1-Ig (same clone as 66205-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66205-1-Ig (Myoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66205-1-Ig (same clone as 66205-1-PBS)

    IHC staining of human heart using 66205-1-Ig (same clone as 66205-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66205-1-Ig (Myoglobin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse tongue using 66205-1-Ig (same clone as 66205-1-PBS)

    IHC staining of mouse tongue using 66205-1-Ig (same clone as 66205-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 66205-1-Ig (Myoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

  • FC experiment of C2C12 using 66205-1-Ig (same clone as 66205-1-PBS)

    FC experiment of C2C12 using 66205-1-Ig (same clone as 66205-1-PBS)

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Myoglobin (66205-1-Ig, Clone:1A4F7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66205-1-PBS in a different storage buffer formulation.

Myoglobin Monoclonal antibody, PBS Only

Myoglobin Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 66205-1-PBS

产品说明书

CloneNo. 1A4F7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

MB, 1A4F7, EC:1.11.1.-, EC:1.7.-.-, Nitrite reductase MB

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

66205-1-PBS targets Myoglobin in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 Myoglobin fusion protein Ag8979 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 myoglobin
别名 MB, 1A4F7, EC:1.11.1.-, EC:1.7.-.-, Nitrite reductase MB
计算分子量 154 aa, 17 kDa
观测分子量 17-18 kDa
GenBank蛋白编号BC014547
基因名称 Myoglobin
Gene ID (NCBI) 4151
RRIDAB_2881596
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP02144
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Recent studies indicated that myoglobin was also expressed in non-muscle tissues. This antibody well recognized endogenous myoglobin in muscle lysates.