验证数据展示
经过测试的应用
| Positive WB detected in | 3T3-L1 cells, BV-2 cells, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
51132-1-AP targets Myocd in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myocardin |
| 别名 | Basic SAP coiled-coil transcription activator 2, Bsac2, BSAC2A, Myocardin, SRF cofactor protein |
| 计算分子量 | 102 kDa |
| 观测分子量 | 95 kDa |
| GenBank蛋白编号 | NM_146386 NM_145136 |
| 基因名称 | Myocd |
| Gene ID (NCBI) | 214384 |
| RRID | AB_3670235 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8VIM5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Myocd (Myocardin) is involved in several processes, including circulatory system development; negative regulation of vascular-associated smooth muscle cell migration; and regulation of signal transduction. Located in chromatin and the nucleus. Orthologous to human MYOCD (myocardin).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Myocd antibody 51132-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomaterials Microenvironment-activatable nanoagent for real-time NIR-II monitoring and targeted therapy of arterial restenosis | ||
J Diabetes Metab Disord miR-148b attenuates ox-LDL-induced phenotypic switching of vascular smooth muscle cells by suppressing KLF4 expression and promoting its ubiquitination. | ||
Int Immunopharmacol Electrical stimulation improves arteriogenic erectile dysfunction by modulating CYLD-mediated macrophage-smooth muscle cell crosstalk. | ||
Am J Physiol Cell Physiol Spatial expression of Myocardin protein in normal and disease states. |
