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验证数据展示

  • WB analysis using 10458-1-AP (same clone as 10458-1-PBS)

    WB analysis using 10458-1-AP (same clone as 10458-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10458-1-AP (Myelin basic protein antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10458-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 10458-1-AP (same clone as 10458-1-PBS)

    IHC staining of mouse brain using 10458-1-AP (same clone as 10458-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10458-1-AP (Myelin basic protein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10458-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MBP antibody included
  • IF Staining of mouse brain using 10458-1-AP (same clone as 10458-1-PBS)

    IF Staining of mouse brain using 10458-1-AP (same clone as 10458-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Myelin basic protein antibody (10458-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10458-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 10458-1-AP (same clone as 10458-1-PBS)

    IF Staining of mouse brain using 10458-1-AP (same clone as 10458-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using Myelin basic protein antibody (10458-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 10458-1-PBS in a different storage buffer formulation.

MBP Polyclonal antibody, PBS Only

MBP Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA
Cat No. 10458-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IF-Fro, ELISA

别名

myelin basic protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MBP Ready-To-Use IHC Kit. MBP primary antibody included.

产品信息

10458-1-PBS targets MBP in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IF-Fro, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0713

Product name: Recombinant human MBP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-171 aa of BC008749

Sequence: MASQKRPSQRHGSKYLATASTMDHARHGFLPRHRDTGILDSIGRFFGGDRGAPKRGSGKDSHHPARTAHYGSLPQKSHGRTQDENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQRPGFGYGGRASDYKSAHKGFKGVDAQGTLSKIFKLGGRDSRSGSPMARR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myelin basic protein
别名 myelin basic protein
计算分子量 33 kDa
观测分子量 14-23 kDa
GenBank蛋白编号BC008749
基因名称 Myelin basic protein
Gene ID (NCBI) 4155
RRIDAB_2250289
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02686
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MBP belongs to the myelin basic protein family. The classic group of MBP isoforms (isoform 4-isoform 14) are the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. MBP has six isoforms. Catalog#10458-1-AP is capable of recognizing multiple isoforms of MBP.

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