验证数据展示
经过测试的应用
| Positive WB detected in | PC-12 cells, C6 cells, COLO 320 cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human ovary tumor tissue, human colon cancer tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10770-1-AP targets MSI2 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag1213 Product name: Recombinant human Musashi-2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC001526 Sequence: MEANGSQGTSGSANDSQHDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADPASVDKVLGQPHHELDSKTIDPKVAFPRRAQPKMVTRTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVMFPPGTRGRARGLPYTMDAFMLGMGMLGYPNFVATYGRGYPGFAPSYGYQFPGFPAAAYGPVAAAAVAAARGSGSNPARPGGFPGANSPGPVADLYGPASQDSGVGNYISAASPQPGSGFGHGIAGPLIATAFTNGYH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | musashi homolog 2 (Drosophila) |
| 别名 | Musashi-2, RNA-binding protein Musashi homolog 2, Musashi 2, MSI2H |
| 计算分子量 | 35 kDa |
| 观测分子量 | 37-40 kDa |
| GenBank蛋白编号 | BC001526 |
| 基因名称 | MSI2 |
| Gene ID (NCBI) | 124540 |
| RRID | AB_2147935 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96DH6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MSI2 is an RNA-binding protein that regulates the expression of target mRNAs at the translation level. It binds to RNA via 2 ribonucleoprotein-type RNA recognition motifs in their N-termini, to consensus motifs in mRNAs to inhibit transcription. It may play a role in the proliferation and maintenance of stem cells in the central nervous system. MSI2 can regulate normal hematopoiesis and promote aggressive myeloid leukemia
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for MSI2 antibody 10770-1-AP | Download protocol |
| IP protocol for MSI2 antibody 10770-1-AP | Download protocol |
| WB protocol for MSI2 antibody 10770-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science Neurodevelopmental protein Musashi-1 interacts with the Zika genome and promotes viral replication. | ||
EMBO J Suppression of miR-199a maturation by HuR is crucial for hypoxia-induced glycolytic switch in hepatocellular carcinoma. | ||
Int J Oncol Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells. | ||
Front Mol Biosci RNA-Binding Protein MSI2 Binds to miR-301a-3p and Facilitates Its Distribution in Mitochondria of Endothelial Cells. | ||
Exp Cell Res Msi2 plays a carcinogenic role in esophageal squamous cell carcinoma via regulation of the Wnt/β-catenin and Hedgehog signaling pathways. |
