验证数据展示
经过测试的应用
Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
Positive Single Cell detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
SINGLE CELL (INTRA) | <0.5ug/test |
SINGLE CELL | <0.5ug/test |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
产品信息
G65063-1-5C targets CD44 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.
经测试应用 | Single Cell (Intra), Single Cell Application Description |
经测试反应性 | Human |
免疫原 | Purified T cells from human lymph nodes 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a, kappa |
抗体类别 | Oligo Conjugate |
产品类型 | Monoclonal |
全称 | MultiPro® 5CFLX Anti-Human CD44 (F10-44-2) |
别名 | CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I |
计算分子量 | 742 aa, 82 kDa |
GenBank蛋白编号 | BC004372 |
基因名称 | CD44 |
Gene ID (NCBI) | 960 |
ENSEMBL Gene ID | ENSG00000026508 |
RRID | AB_3673907 |
偶联类型 | 5CFLX |
完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGAACAGCGTGCCGATTCCCATATAAGAAA |
条形码序列 | AACAGCGTGCCGATT |
形式 | Liquid |
UNIPROT ID | P16070 |
储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3 |
储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.
Protocols
Our current MultiPro staining protocol for the cocktail | Download protocol |
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |