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Validation Data Gallery

  • Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:800 and Multi-rAb® CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

    Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:800 and Multi-rAb® CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

  • Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, green) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, red). Multi-rAb® CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb® CoraLite® Plus 594 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM004, 1:500) .

    Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, green) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, red). Multi-rAb® CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb® CoraLite® Plus 594 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM004, 1:500) .

  • Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, green) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, red). Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004, 1:500) were used for detection.

    Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, green) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, red). Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004, 1:500) were used for detection.

  • Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, green) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, red). Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004, 1:500) were used for detection.

    Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, green) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, red). Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004, 1:500) were used for detection.

  • Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, green) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, red). Multi-rAb® CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb® CoraLite® Plus 594 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM004, 1:500) .

    Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, green) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, red). Multi-rAb® CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb® CoraLite® Plus 594 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM004, 1:500) .

  • 1X10^6 MOLT4 were surface stained with 0.2 ug Anti-Human CD8 (65204-1-Ig, Clone: UCHT4) and Mouse IgG2a Isotype Control 66360-2-Ig. Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM004 was used at 1:500 for detection.

    1X10^6 MOLT4 were surface stained with 0.2 ug Anti-Human CD8 (65204-1-Ig, Clone: UCHT4) and Mouse IgG2a Isotype Control 66360-2-Ig. Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM004 was used at 1:500 for detection.

Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)

Multi-rAb® Goat Anti-Mouse Recombinant Secondary Antibody (H+L) is a mixture of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on the mouse IgG heavy and light chains.
Cat No. RGAM004

Applications

IF, FC

Conjugate

CoraLite® Plus 594

Host

Goat

Reactivity

Mouse

规格价格库存

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Product Information

RGAM004 targets Mouse IgG (H+L) in FC and IF. It can bind to all mouse IgG isotypes, mouse IgM and IgA as well as rat IgG. It shows minimal reactivity for rabbit, human, pig and bovine IgGs.

Concentration 0.5 mg/mL
Applications IF, FC
Host Species Goat
Species Reactivity Mouse
Physical State Liquid
Suggested Dilution Range 1:200-1:1000 for IF and FC
Purity The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads.
Storage Buffer PBS with 50% glycerol, 10 mg/mL BSA, 0.1% ProClin 300, pH 7.4.
Storage Instructions Store at -20°C. Stable for one year after shipment.
FluorophoreCoraLite® Plus 594, Amax=588 nm, Emax=604 nm
ClonalityMulticlonal recombinant
RRIDAB_3073502
NoteThis product is for research use only, not for diagnostic or therapeutic use.

背景介绍

Multi-rAb® Recombinant Secondary Antibodies are mixtures of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on the same IgG. Each recombinant clone in the final multiclonal mixture is carefully selected after rigorous characterization and screening to ensure the highest level of performance. ​The selected clones are mixed in various combinations, each of which is then functionally characterized in multiple applications to select the final multiclonal mixture with optimal performance. Each lot of Multi-rAb® Secondary Antibodies is produced by mixing the same high performing recombinant clones every time to ensure that they have high batch-to-batch consistency, which in turn results in high reproducibility of experimental data.

Publications

Title

Adv Mater

Mechano-Iontronic Hydrogels Generating Biomimetic Endogenous Bioelectricity for Promoting Cartilage Regeneration.

Authors - Longwei Li
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Drug Resist Updat

ZBP1 antagonizes MRE11-mediated DNA end resection and confers synthetic lethality to PARP inhibition in ovarian cancer.

Authors - Shen-Nan Shi
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Autophagy

African swine fever virus I10L protein inhibits autolysosome formation by disrupting RAB7-HOPS complex-dependent SNARE complex assembly.

Authors - Meng Chen
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Nat Commun

Self-polymerized polyphenol-based platform for the management of dry eye pathogenesis.

Authors - Zixin Wang
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J Adv Res

Modulating GRP75 to restore calcium homeostasis: a novel neuroprotective strategy of CRSE6(#) in Parkinson's disease models.

Authors - Xiaofang Wang
View Article

Metabolism

Setmelanotide alleviates diabetes-associated cognitive impairment by interrupting the binding of ICAM-1 and JIP1 in endothelial cells of the blood-brain barrier.

Authors - Shi-Di Wu
View Article
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