Mouse sVEGFR1/Flt-1 ELISA Kit
Validation Data Gallery
Product Information
KE10069 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse sVEGFR1/Flt-1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse sVEGFR1/Flt-1. The assay recognizes mouse sVEGFR1/Flt-1. An antibody specific for mouse sVEGFR1/Flt-1 has been pre-coated onto the microwells. The mouse sVEGFR1/Flt-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse sVEGFR1/Flt-1 is added to detect the captured mouse sVEGFR1/Flt-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse sVEGFR1/Flt-1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.72 pg/mL |
| Range | 156-10000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 14254 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 97% | 86%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 5,296.1 | 268.7 | 5.1 |
| 2 | 20 | 1,231.9 | 31.8 | 2.6 |
| 3 | 20 | 326.0 | 13.4 | 4.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 5,241.8 | 314.1 | 6.0 |
| 2 | 24 | 1,264.9 | 86.8 | 6.9 |
| 3 | 24 | 348.5 | 20.3 | 5.8 |
Background Information
Vascular endothelial growth factor receptor-1 (VEGFR-1, FLT-1) is a receptor tyrosine kinase belonging to the VEGFR family. VEGF is a key regulator of physiological angiogenesis and has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders and other conditions. The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2. Both the two receptors have seven immunoglobulin-like repeats in the extracellular domain, a single transmembrane region and a tyrosine kinase domain. VEGFR-1 binds VEGFA, PIGF and VEGFB, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Besides the membrane-bound form, VEGFR-1 can also exist as a soluble form (sVEGFR-1) generated by alternative splicing of VEGFR-1 mRNA.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:2.7.10.1, Embryonic receptor kinase 2, Emrk2, Flt, Flt1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
EBioMedicine H3K27me3-modulated Hofbauer cell BMP2 signalling enhancement compensates for shallow trophoblast invasion in preeclampsia | ||
Placenta Abnormal placental development induced by repeated cesarean sections: Investigating an animal model of placenta accreta spectrum disorders | ||
Toxicol Sci E-cigarette exposure during pregnancy impairs uterine artery blood flow and feto-placental function. | ||
Cell Mol Life Sci BMP6 as a therapeutic target for preeclampsia: enhancing trophoblast invasion and vascular mimicry. |
