Mouse/Rat HGF ELISA Kit
Cat no : KE10079
Synonyms
Hepatocyte growth factor, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain, Hepatopoietin-A, Scatter factor
Validation Data Gallery
Product Information
KE10079 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse/rat HGF ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat HGF. The assay recognizes mouse/rat HGF. An antibody specific for mouse/rat HGF has been pre-coated onto the microwells. The mouse/rat HGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse/rat HGF is added to detect the captured mouse/rat HGF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse/Rat HGF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Tissue lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 15234 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 106% | 96%-115% |
| Rat serum | 97% | 76%-106% |
| Cell culture supernatants | 107% | 93%-125% |
| Tissue lysates | 95% | 81%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,124.6 | 42.3 | 2.0 |
| 2 | 20 | 521.5 | 12.4 | 2.4 |
| 3 | 20 | 133.7 | 4.1 | 3.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,110.2 | 29.9 | 1.4 |
| 2 | 24 | 516.5 | 17.2 | 3.3 |
| 3 | 24 | 126.2 | 6.2 | 4.9 |
Background Information
HGF (hepatocyte growth factor) is a potent mitogen for hepatocytes, also known as hepatopoietin A (HBGA). HGF and its receptor MET are involved in cell proliferation, movement, differentiation, invasion, angiogenesis, and apoptosis by activating multiple downstream signaling pathways. HGF/cMet also can serve as biomarkers for gastrointestinal cancers. HGF regulates inflammation including collagen-induced arthritis, autoimmune neuroinflammation, inflammatory bowel disease and airway inflammation. Elevated levels of HGF of tumors and plasma were observed such as in invasive breast carcinoma, glioma, multiple myeloma and sarcomas.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Hepatocyte growth factor, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain, Hepatopoietin-A, Scatter factor |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Biochem Pharmacol Monocyte-derived macrophage SQSTM1/p62 deficiency impairs liver repair and regeneration | ||
Biomaterials Topology scaffolds-enhanced paracrine of BMSCs through mechanotransduction-related metabolism reprogramming for burn wounds healing |
