Mouse VEGF ELISA Kit
Cat no : KE10009
Synonyms
Vegfa, L-VEGF , N-VEGF, Vascular Endothelial Growth Factor, vascular endothelial growth factor A
Validation Data Gallery
Product Information
KE10009 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse VEGF ELISA kit is to be used to detect and quantify protein levels of endogenous mouse VEGF. The assay recognizes mouse VEGF. An antibody specific for mouse VEGF has been pre-coated onto the microwells. The mouse VEGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse VEGF is added to detect the captured mouse VEGF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse VEGF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.0 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 22339 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 93% | 74%-110% |
| Cell culture supernatants | 94% | 92%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 37.0 | 2.5 | 3.4 |
| 2 | 20 | 118.0 | 9.0 | 3.8 |
| 3 | 20 | 369.0 | 63.6 | 8.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 39.0 | 4.5 | 5.7 |
| 2 | 24 | 128.0 | 7.1 | 2.8 |
| 3 | 24 | 390.0 | 31.9 | 4.1 |
Background Information
Vascular endothelial growth factor (VEGF), is a signal protein produced by cells that stimulates vasculogenesis and angiogenesis. It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate such as in hypoxic conditions. Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus. The activities of VEGF are not limited to the vascular system; VEGF plays a role in normal physiological functions such as bone formation, hematopoiesis, wound healing, and development. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. VEGF is upregulated in many known tumors and its expression is correlated with tumor stage and progression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Vegfa, L-VEGF , N-VEGF, Vascular Endothelial Growth Factor, vascular endothelial growth factor A |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Nat Nanotechnol Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades. | ||
Bone Res Osteopontin deficiency promotes cartilaginous endplate degeneration by enhancing the NF-κB signaling to recruit macrophages and activate the NLRP3 inflammasome | ||
Theranostics Cardio-renal Exosomes in Myocardial Infarction Serum Regulate Proangiogenic Paracrine Signaling in Adipose Mesenchymal Stem Cells. | ||
Endocrinology Dietary intake regulates white adipose tissues angiogenesis via liver fibroblast growth factor 21 in male mice. | ||
Front Behav Neurosci Regular Low-Intensity Exercise Prevents Cognitive Decline and a Depressive-Like State Induced by Physical Inactivity in Mice: A New Physical Inactivity Experiment Model. | ||
Heliyon Ginseng-Sanqi-Chuanxiong (GSC) extracts attenuate d-galactose-induced vascular aging in mice via inhibition of endothelial progenitor cells senescence |
