Mouse VCAM-1/CD106 ELISA Kit
Validation Data Gallery
Product Information
KE10038 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The VCAM-1 ELISA kit is to be used to detect and quantify protein levels of endogenous VCAM-1. The assay recognizes mouse VCAM-1. An antibody specific for VCAM-1 has been pre-coated onto the microwells. The VCAM-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for VCAM-1 is added to detect the captured VCAM-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse VCAM-1/CD106 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 22329 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 102% | 96%-118% |
| Cell culture supernatants | 92% | 85%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 766.0 | 23.0 | 3.0 |
| 2 | 20 | 412.4 | 6.3 | 1.5 |
| 3 | 20 | 317.8 | 6.5 | 2.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 689.0 | 48.9 | 7.1 |
| 2 | 24 | 375.9 | 28.4 | 7.6 |
| 3 | 24 | 283.9 | 20.0 | 7.0 |
Background Information
Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM-1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. The VCAM-1/integrin VLA4 (α4β1) interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM-1 is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells. Soluble VCAM-1 (sVCAM-1) has been found in addition to membrane-bound VCAM-1.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD106, Vcam1, VCAM-1, Vcam 1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Pharmacol Res Zedoarondiol inhibits atherosclerosis by regulating monocyte migration and adhesion via CXCL12/CXCR4 pathway. | ||
J Extracell Vesicles Endothelial cell-derived extracellular vesicles expressing surface VCAM1 promote sepsis-related acute lung injury by targeting and reprogramming monocytes | ||
Int J Biol Macromol Nattokinase attenuates endothelial inflammation through the activation of SRF and THBS1 |
