Mouse TSLP ELISA Kit
Validation Data Gallery
Product Information
KE10083 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse TSLP ELISA kit is to be used to detect and quantify protein levels of endogenous mouse TSLP. The assay recognizes mouse TSLP. An antibody specific for mouse TSLP has been pre-coated onto the microwells. The mouse TSLP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse TSLP is added to detect the captured mouse TSLP protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
| Product name | Mouse TSLP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.3 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 53603 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 86% | 73%-115% |
| Cell culture supernatants | 102% | 75%-123% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 118.7 | 6.7 | 5.6 |
| 2 | 20 | 207.8 | 12.1 | 5.8 |
| 3 | 20 | 376.4 | 11.0 | 2.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 118.9 | 7.0 | 5.9 |
| 2 | 24 | 204.2 | 12.0 | 5.9 |
| 3 | 24 | 355.4 | 32.3 | 9.1 |
Background Information
Thymic stromal lymphopoietin (TSLP) is a 24 kDa monomeric glycoprotein that was originally identified as a product of a thymic stromal cell line. TSLP mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c+ dendritic cells. It can induce allergic inflammation by directly activating mast cells. TSLP is expressed in a number of tissues including heart, liver and prostate. Human TSLP shares only 38% aa sequence identity with mouse TSLP in the mature region. TSLP has been shown to expand the CD4+ subset of lymphocytes and thymocytes and thus regulate CD4+ homeostasis. Overexpression of TSLP causes imbalances in lymphopoiesis and myelopoiesis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | thymic stromal lymphopoietin, Tslp |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Immunol Lett Role of G-protein-coupled estrogen receptor in the pathogenesis of chronic asthma |
