Mouse TNF RII/TNFRSF1B ELISA Kit
Sensitivity
3.3 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
KE10116 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse TNF RII/TNFRSF1B ELISA kit is to be used to detect and quantify protein levels of endogenous mouse TNF RII/TNFRSF1B. The assay recognizes mouse TNF RII/TNFRSF1B. An antibody specific for mouse TNF RII/TNFRSF1B has been pre-coated onto the microwells. The mouse TNF RII/TNFRSF1B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse TNF RII/TNFRSF1B is added to detect the captured mouse TNF RII/TNFRSF1B protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse TNF RII/TNFRSF1B ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 3.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 21938 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 92% | 76%-115% |
| Cell culture supernatant | 82% | 70%-104% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 540.4 | 20.8 | 3.8 |
| 2 | 20 | 125.0 | 6.0 | 4.8 |
| 3 | 20 | 31.4 | 1.6 | 5.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 544.4 | 19.1 | 3.5 |
| 2 | 24 | 136.0 | 4.5 | 3.3 |
| 3 | 24 | 33.7 | 3.3 | 9.7 |
Background Information
TNFRSF1B, also known as TNFR2 or CD120b or p75, is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1A. Tumor necrosis factor (TNF, TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis. TNF signals through TNFRSF1A and TNFRSF1B. TNFRSF1A is widely expressed, whereas TNFRSF1B exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells. Both TNFRSF1A and TNFRSF1B can also exist in soluble forms, probably derived by proteolytic cleavage from the cell surface forms. Various defects in the TNFRSF1B pathway, due to polymorphisms in the TNFRSF1B gene, upregulated expression of TNFRSF1B and TNFRSF1B shedding, have been implicated in the pathology of several autoimmune disorders.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD120b,TNFR2,Tnfrsf1b,p75,TNF alphaR2 |