Speedy™ Mouse Serpin E1/PAI-1 One-Step ELISA Kit
Sensitivity
2.9 pg/mL
Range
23.4-1500 pg/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse Serpin E1/PAI-1 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.9 pg/mL |
| Range | 23.4-1500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 18787 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 101% | 85%-109% |
| Cell culture supernatant | 99% | 97%-102% |
| Tissue lysate | 104% | 101%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 803.9 | 32.1 | 4.0 |
| 2 | 8 | 218.1 | 12.7 | 5.8 |
| 3 | 8 | 116.2 | 5.8 | 5.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 818.9 | 66.3 | 8.1 |
| 2 | 16 | 211.3 | 11.8 | 5.6 |
| 3 | 16 | 114.1 | 4.8 | 4.2 |
Background Information
Serpin E1, also known as plasminogen activator inhibitor 1 (PAI-1), is a protein that belongs to the serpin family of serine protease inhibitors. It is primarily produced by endothelial cells and plays a crucial role in the regulation of fibrinolysis by inhibiting the activity of plasminogen activators, such as tissue plasminogen activator (tPA) and urokinase (uPA). Serpin E1 is involved in a variety of physiological and pathological processes, including fibrinolysis, fibrosis, angiogenesis, wound healing, and the invasion and metastasis of tumor cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PAI-1, Serpine1, PAI 1, PAI1, Plasminogen activator inhibitor 1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
