Mouse SCF ELISA Kit
Sensitivity
0.5 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Mouse
Cat no : KE10047
Synonyms
Clo, Con, contrasted, Gb, kit ligand, Kitl, Kitlg, Mgf, SCF, SF, Sl, SLF, Steel
Validation Data Gallery
Product Information
KE10047 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse SCF ELISA kit is to be used to detect and quantify protein levels of endogenous mouse SCF. The assay recognizes mouse SCF. An antibody specific for mouse SCF has been pre-coated onto the microwells. The mouse SCF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse SCF is added to detect the captured mouse SCF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse SCF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 17311 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 90% | 70%-109% |
| Cell culture supernatants | 101% | 95%-109% |
| Mouse urine | 91% | 79%-110% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 904.4 | 18.1 | 2.0 |
| 2 | 20 | 249.1 | 4.4 | 1.8 |
| 3 | 20 | 88.1 | 3.0 | 3.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 924.5 | 36.2 | 3.9 |
| 2 | 24 | 247.3 | 10.9 | 4.4 |
| 3 | 24 | 93.5 | 3.9 | 4.2 |
Background Information
Stem cell factor (SCF) also known as mast cell growth factor, steel factor, and kit ligand, is a hematopoietic growth factor. SCF is a critical protein with key roles in the cell such as hematopoiesis, gametogenesis and melanogenesis. SCF activates multiple signal pathways by binding with c-kit receptor. SCF and its receptor, c-kit ligand (KL), are up-regulated in particular human malignancies including gastrointestinal stromal tumor (GISTs), breast cancer, hematopoietic cell, myeloid leukaemia, and glioma. The SCF/c-KIT system also plays a relevant role in cell fate decision, and its activity is widely known to control cell proliferation, differentiation, survival and apoptosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Clo, Con, contrasted, Gb, kit ligand, Kitl, Kitlg, Mgf, SCF, SF, Sl, SLF, Steel |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
