Mouse/Rat Osteopontin/OPN ELISA Kit

Sensitivity

2.5 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Mouse/Rat

Cat No : KE10046

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Synonyms

AA960535, AI790405, Apl 1, BNSP, Bsp, BSPI, Eta, ETA 1, minopontin, OP, Opn, Opnl, Osteopontin, Ric, secreted phosphoprotein 1, Spp 1, Spp1



Product Information

KE10046 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Osteopontin ELISA kit is to be used to detect and quantify protein levels of endogenous Osteopontin. The assay recognizes mouse/rat Osteopontin. An antibody specific for Osteopontin has been pre-coated onto the microwells. The Osteopontin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Osteopontin is added to detect the captured Osteopontin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .

Product name Mouse/Rat Osteopontin/OPN ELISA Kit
Tests 1 X 96 well plate
Sample type Mouse serum, Mouse plasma, Mouse cell cuture supernatants, Mouse urine, Rat serum, Rat plasma, Cell cuture supernatants
Assay type Sandwich
Sensitivity 2.5 pg/mL
Range 15.6-1000 pg/mL
Reactivity Mouse/Rat
Tested applications Sandwich ELISA
Gene ID (NCBI) 20750

Recovery

Sample TypeAverageRange
Mouse serum88%81%-95%
Mouse cell cuture supernatants103%85%-120%
Mouse urine100%80%-120%
Rat cell cuture supernatants93%84%-108%
Rat serum87%81%-93%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12025.50.62.4
220108.32.01.8
320429.98.72.0

InterAssay

Samplenmean ( pg/mL)SDCV%
12427.61.65.8
224114.44.23.7
324444.417.43.9

Background Information

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues. OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms AA960535, AI790405, Apl 1, BNSP, Bsp, BSPI, Eta, ETA 1, minopontin, OP, Opn, Opnl, Osteopontin, Ric, secreted phosphoprotein 1, Spp 1, Spp1