Speedy™ Mouse/Rat HGF One-Step ELISA Kit
Sensitivity
7.8 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Mouse/Rat
Cat no : SE60014
Synonyms
Hepatocyte growth factor, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain, Hepatopoietin-A, Scatter factor
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse/Rat HGF One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Mouse suerm, Mouse plasma, Rat serum, Rat plasma, Cell culture supernatant, Tissue homogenate |
| Assay type | Sandwich |
| Sensitivity | 7.8 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 15234 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 124% | 122%-128% |
| Rat serum | 121% | 115%-126% |
| Cell culture supernatant | 107% | 88%-127% |
| Tissue homogenate | 120% | 116%-128% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,032.5 | 38.5 | 1.9 |
| 2 | 8 | 518.4 | 13.2 | 2.5 |
| 3 | 8 | 257.8 | 5.5 | 2.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,074.0 | 103.6 | 5.0 |
| 2 | 16 | 518.2 | 9.7 | 1.9 |
| 3 | 16 | 256.1 | 7.0 | 2.7 |
Background Information
HGF (hepatocyte growth factor) is a potent mitogen for hepatocytes, also known as hepatopoietin A (HBGA). HGF and its receptor MET are involved in cell proliferation, movement, differentiation, invasion, angiogenesis, and apoptosis by activating multiple downstream signaling pathways. HGF/cMet also can serve as biomarkers for gastrointestinal cancers. HGF regulates inflammation including collagen-induced arthritis, autoimmune neuroinflammation, inflammatory bowel disease and airway inflammation. Elevated levels of HGF of tumors and plasma were observed such as in invasive breast carcinoma, glioma, multiple myeloma and sarcomas.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Hepatocyte growth factor, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain, Hepatopoietin-A, Scatter factor |
