Mouse/Rat FGF-21 ELISA Kit
Validation Data Gallery
Product Information
KE10095 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse/Rat FGF-21 ELISA kit is to be used to detect and quantify protein levels of endogenous FGF-21. The assay recognizes mouse/rat FGF-21. An antibody specific for FGF-21 has been pre-coated onto the microwells. The FGF-21 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for FGF-21 is added to detect the captured FGF-21 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse/Rat FGF-21 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 19.3 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 56636 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 110% | 91%-120% |
| Rat serum | 91% | 83%-100% |
| Cell culture supernatant | 110% | 98%-120% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,033.8 | 34.9 | 3.4 |
| 2 | 20 | 258.7 | 11.2 | 4.3 |
| 3 | 20 | 62.5 | 4.4 | 7.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,040.3 | 61.0 | 5.9 |
| 2 | 24 | 250.3 | 18.5 | 7.4 |
| 3 | 24 | 125.3 | 10.4 | 8.3 |
Background Information
Fibroblast growth factor 21 (FGF21) is a peptide hormone that is expressed across several tissues, predominantly in liver and adipocyte tissues, and, to a lower extent, in the pancreas, skeletal muscle, heart, kidneys and testes. FGF21 plays a role in regulating adaptation to various metabolic abnormalities. In the liver, FGF21 plays an important role in the regulation of fatty acid oxidation both in the fasted state and in mice consuming a high-fat, low-carbohydrate ketogenic diet. FGF21 also regulates fatty acid metabolism in mice consuming a diet that promotes hepatic lipotoxicity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Fgf21, fibroblast growth factor 21 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Control Release Sorbitol-responsive in situ gel improves rat ischemic hearts: Supporting mesenchymal stem cell survival and enhancing paracrine effect | ||
Nutrients Lactiplantibacillus plantarum Strain 06CC2 Attenuates Fat Accumulation and Modulates the Gut Microbiota in a Mouse Model of Early-Stage Diet-Induced Obesity. | ||
Clin Exp Hypertens FUNDC2 contributes to hypertensive vascular remodeling by regulating mitochondrial dynamics and ferroptosis in perivascular adipose tissue. | ||
Phytomedicine Ginsenoside Rg2 ameliorates acute cold exposure/rewarming-induced myocardial injury via modulating HMGB1/TLR4/NF-κB and PGC-1α signaling pathways: Role of SIRT1. | ||
J Ethnopharmacol Inonotus obliquus (Ach. ex Pers.) Pilát aqueous extract alleviates acute cold exposure/rewarming-induced myocardial injury by regulating mitochondrial dynamics via liver kinase B1/adenosine monophosphate-activated protein kinase/peroxisome proliferator-activated receptor gamma coactivator-1 alpha signaling pathway activation. | ||
Animal Model Exp Med Metabolic and biochemical profiling reveals phenotypic heterogeneity in Zucker diabetic fatty rats. |
