Mouse Osteoprotegerin/TNFRSF11B ELISA Kit
Validation Data Gallery
Product Information
KE10058 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Osteoprotegerin/TNFRSF11B ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Osteoprotegerin/TNFRSF11B. The assay recognizes mouse Osteoprotegerin/TNFRSF11B. An antibody specific for mouse Osteoprotegerin/TNFRSF11B has been pre-coated onto the microwells. The mouse Osteoprotegerin/TNFRSF11B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse Osteoprotegerin/TNFRSF11B is added to detect the captured mouse Osteoprotegerin/TNFRSF11B protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Osteoprotegerin/TNFRSF11B ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell Culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.04 pg/mL |
| Range | 31.3-2000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 18383 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse Serum | 99% | 73%-122% |
| Cell Culture supernatants | 97% | 73%-114% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 48.0 | 1.7 | 3.5 |
| 2 | 20 | 207.5 | 4.9 | 2.3 |
| 3 | 20 | 859.6 | 25.6 | 3.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 46.6 | 1.8 | 3.9 |
| 2 | 24 | 195.6 | 9.3 | 4.7 |
| 3 | 24 | 806.4 | 36.0 | 4.5 |
Background Information
Osteoprotegerin (OPG), also known as TNFRSF11B or OCIF, is a member of the tumor necrosis factor receptor super family (TNFRSF). It is a secreted glycoprotein that inhibits osteoclastogenesis and is involved in the regulation of bone density. Osteoprotegerin acts as decoy receptor for receptor activator of nuclear factor-kappa B ligand (RANKL) and neutralizes its function in osteoclastogenesis. Osteoprotegerin may function as a soluble decoy receptor for tumor necrosis factor (TNF)-related apoptosis inducing ligand (TRAIL) and inhibits TRAIL-induced apoptosis. TRAIL blocks osteoprotegerin mediated inhibition of osteoclastogenesis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Tnfrsf11b, OCIF, Opg, Osteoprotegerin, PDB5 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Clin Nutr Eicosapentaenoic acid supplementation modulates the osteoblast/osteoclast balance in inflammatory environments and protects against estrogen deficiency-induced bone loss in mice | ||
Polymers (Basel) Three-Dimensional Bioprinting of Strontium-Modified Controlled Assembly of Collagen Polylactic Acid Composite Scaffold for Bone Repair | ||
Immun Inflamm Dis Combined application of mesenchymal stem cells and different glucocorticoid dosing alleviates osteoporosis in SLE murine models | ||
Int J Mol Sci HuR Knockdown in MLO-Y4 Osteocyte-like Cells Elevates OPG Expression and Suppresses Osteoclastogenesis In Vitro. |
