Mouse MCP-1 ELISA Kit
Sensitivity
1.2 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
KE10123 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse MCP-1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse MCP-1. The assay recognizes mouse MCP-1. An antibody specific for mouse MCP-1 has been pre-coated onto the microwells. The mouse MCP-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse MCP-1 is added to detect the captured mouse MCP-1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse MCP-1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 1.2 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20296 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 110% | 79%-129% |
| Cell culture supernatant | 95% | 85%-117% |
| Tissue lysate | 100% | 92%-115% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 509.8 | 10.8 | 2.1 |
| 2 | 20 | 119.1 | 3.2 | 2.7 |
| 3 | 20 | 29.1 | 1.5 | 5.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 508.0 | 33.1 | 6.5 |
| 2 | 24 | 122.5 | 6.6 | 5.4 |
| 3 | 24 | 29.8 | 1.9 | 6.2 |
Background Information
Monocyte chemotactic protein 1 (Mcp1; also known as Ccl2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ccl2,MCP1,MCP-1,C-C motif chemokine 2,CCL 2 |