Mouse LAG-3/CD223 ELISA Kit
Sensitivity
0.10 ng/mL
Range
0.313-20 ng/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
KE10189 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse LAG-3/CD223 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse LAG-3/CD223. The assay recognizes mouse LAG-3/CD223. An antibody specific for mouse LAG-3/CD223 has been pre-coated onto the microwells. The mouse LAG-3/CD223 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse LAG-3/CD223 is added to detect the captured mouse LAG-3/CD223 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse LAG-3/CD223 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.10 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16768 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatant | 95% | 80%-119% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 12.64 | 0.68 | 5.37 |
| 2 | 8 | 2.45 | 0.05 | 1.96 |
| 3 | 8 | 1.31 | 0.03 | 2.56 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 12.29 | 0.90 | 7.31 |
| 2 | 16 | 2.42 | 0.07 | 2.92 |
| 3 | 16 | 1.31 | 0.03 | 2.63 |
Background Information
LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. Its soluble form, sLAG-3, produced via alternative splicing and proteolytic cleavage of the extracellular domain, is a natural high-affinity ligand for MHC class II.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD223, LAG-3, LAG 3, Lag3, Ly66 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
