Mouse IL-4RA/CD124 ELISA Kit
Sensitivity
0.4 pg/mL
Range
23.4-1500 pg/mL
Reactivity
Mouse
Cat no : KE10188
Synonyms
CD124, IL-4 receptor subunit alpha, Il4r, IL-4R subunit alpha, Il4ra
Validation Data Gallery
Product Information
KE10188 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-4RA/CD124 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-4RA/CD124. The assay recognizes mouse IL-4RA/CD124. An antibody specific for mouse IL-4RA/CD124 has been pre-coated onto the microwells. The mouse IL-4RA/CD124 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse IL-4RA/CD124 is added to detect the captured mouse IL-4RA/CD124 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-4RA/CD124 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Cell lysate, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.4 pg/mL |
| Range | 23.4-1500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16190 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 99% | 92%-105% |
| Cell culture supernatant | 94% | 90%-103% |
| Lysate | 92% | 80%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 644.0 | 13.5 | 2.1 |
| 2 | 8 | 179.2 | 10.3 | 5.7 |
| 3 | 8 | 88.8 | 3.6 | 4.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 696.4 | 65.3 | 9.4 |
| 2 | 16 | 185.2 | 11.5 | 6.2 |
| 3 | 16 | 89.6 | 3.7 | 4.1 |
Background Information
IL-4Rα (IL-4 receptor alpha chain, also known as CD124) is an important member of the cytokine receptor family, primarily involved in immune responses and cellular signal transduction. It is a single-pass transmembrane protein that binds to IL-4 and IL-13 through its extracellular domain, activating the JAK/STAT signaling pathway to regulate cell proliferation, differentiation, and function. IL-4Rα is expressed in various immune cells (such as T cells, B cells, and macrophages) and is crucial for Th2 cell-mediated immune responses. In allergic diseases (such as asthma and atopic dermatitis), IL-4Rα plays a key role by promoting the production of IgE antibodies and the activation of inflammatory cells. Additionally, IL-4Rα is involved in the regulation of autoimmune diseases and the tumor microenvironment. Studying the signaling pathways and functional mechanisms of IL-4Rα helps to develop new therapeutic strategies for allergies, inflammation, and cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD124, IL-4 receptor subunit alpha, Il4r, IL-4R subunit alpha, Il4ra |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
