Mouse IL-1ra/IL-1F3 ELISA Kit
Validation Data Gallery
Product Information
KE10052 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-1ra ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1ra. The assay recognizes mouse IL-1ra. An antibody specific for IL-1ra has been pre-coated onto the microwells. The IL-1ra protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-1ra is added to detect the captured IL-1ra protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-1ra/IL-1F3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 3.0 pg/mL |
| Range | 15.6-1000 pg/mL, 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16181 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 99% | 78%-120% |
| cell culture supernatants | 99% | 79%-118% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 100.4 | 2.4 | 2.4 |
| 2 | 20 | 190.5 | 4.8 | 2.5 |
| 3 | 20 | 605.6 | 18.4 | 3.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 103.0 | 3.0 | 3.0 |
| 2 | 24 | 195.9 | 7.8 | 4.0 |
| 3 | 24 | 598.0 | 15.5 | 2.6 |
Background Information
IL-1Ra, also known as IL-1F3, is a member of the interleukin 1 cytokine family. It is a receptor antagonist of IL-1, can competitively bind with IL-1R1 thereby blocking cell activation by the cytokine. IL-1Ra is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, and is a natural inhibitor of the pro-inflammatory effect of IL1β. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses, particularly in the acute phase of infection and inflammation. As a potent immune/inflammatory molecule, IL-1Ra is tightly associated with osteomyelitis, rheumatoid arthritis, traumatic brain injury (TBI) and stroke. Recently, an increasing number of studies proved that IL-1Ra is also actively involved in tumor progression, such as high expression IL-1Ra increased the risk of bladder cancer and non-cardia gastric carcinoma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | F630041P17Rik, IL 1ra, IL-1F3, IL-1ra, Il1rn |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Immunity CINTER-seq: Chemical profiling reveals interaction-dependent cell landscapes and gene signatures in vivo. | ||
J Control Release SpyTag-PEGylated probiotics delivering IL-1Ra modulate gut-lung crosstalk to mitigate septic lung injury. |
