Mouse IL-12/IL-23 p40 ELISA Kit
Validation Data Gallery
Product Information
KE10068 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-12/IL-23 p40 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-12/IL-23 p40. The assay recognizes mouse IL-12/IL-23 p40. An antibody specific for mouse IL-12/IL-23 p40 has been pre-coated onto the microwells. The mouse IL-12/IL-23 p40 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-12/IL-23 p40 is added to detect the captured mouse IL-12/IL-23 p40 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-12/IL-23 p40 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16160 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 93% | 75%-121% |
| Cell culture supernatants | 93% | 75%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 427.0 | 18.2 | 4.3 |
| 2 | 20 | 100.4 | 2.4 | 2.4 |
| 3 | 20 | 27.9 | 0.8 | 3.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 424.4 | 19.6 | 4.6 |
| 2 | 24 | 95.4 | 3.5 | 3.7 |
| 3 | 24 | 28.0 | 1.3 | 4.7 |
Background Information
IL-12p40 is known as a component of the bioactive cytokines interleukin IL-12 and IL-23, which can exist in a monomeric or homodimeric form. IL-12p40 is produced primarily by activated inflammatory cells including macrophages, neutrophils, microglia and dendritic cells (DCs). The most widely appreciated function of IL-12p40 is to provide a negative feedback loop by competitively binding to the IL-12 receptor. Additionally, IL-12p40 can also act as a chemoattractant for macrophages and promote the migration of bacterially stimulated dendritic cells. It is also associated with several pathogenic inflammatory responses such as silicosis, graft rejection.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il 12b, Il 12p40, Il12b, Il12p40, interleukin 12b, p40 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Nanobiotechnology Photosensitive and dual-targeted chromium nanoparticle delivering small interfering RNA YTHDF1 for molecular-targeted immunotherapy in liver cancer | ||
Imeta Akkermansia muciniphila-derived hypoacylated rough-type lipopolysaccharides alleviate diet-induced obesity via activation of TLR4-IL-23-IL-22 immune axis. | ||
J Exp Clin Cancer Res LDRT combined with anti-PD-1 promotes tumor regression in head and neck squamous cell carcinoma through tumor metaprogram evolution and immune cell reprogramming. |
