Mouse IL-1 alpha ELISA Kit
Cat no : KE10098
Synonyms
Il 1a, IL1, IL-1, IL1 alpha, IL-1 alpha, IL-1 α, Il1a, IL1alpha, IL-1α, interleukin 1 alpha, Interleukin-1 alpha, mouse IL-1 alpha
Validation Data Gallery
Product Information
KE10098 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-1 alpha ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-1 alpha. The assay recognizes mouse IL-1 alpha. An antibody specific for mouse IL-1 alpha has been pre-coated onto the microwells. The mouse IL-1 alpha protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-1 alpha is added to detect the captured mouse IL-1 alpha protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-1 alpha ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.4 pg/mL |
| Range | 4.7-300 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16175 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 100% | 76%-124% |
| Cell culture supernatant | 85% | 71%-96% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 135.5 | 3.8 | 2.8 |
| 2 | 20 | 30.1 | 0.7 | 2.4 |
| 3 | 20 | 7.5 | 0.2 | 2.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 133.1 | 6.2 | 4.7 |
| 2 | 24 | 29.6 | 1.3 | 4.3 |
| 3 | 24 | 6.8 | 0.5 | 6.7 |
Background Information
Interleukin-1α (IL-1 Alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 Alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. IL-1 Alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 Alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il 1a, IL1, IL-1, IL1 alpha, IL-1 alpha, IL-1 α, Il1a, IL1alpha, IL-1α, interleukin 1 alpha, Interleukin-1 alpha, mouse IL-1 alpha |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Exp Med Tet2 deficiency-induced expansion of monocyte-derived macrophages promotes liver fibrosis. |
