Mouse IL-1 alpha ELISA Kit

Sensitivity

0.4 pg/mL

Range

4.7-300 pg/mL

Reactivity

Mouse

Cat No : KE10098

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Synonyms

Il 1a, IL1, IL-1, IL1 alpha, IL-1 alpha, IL-1 α, Il1a, IL1alpha, IL-1α, interleukin 1 alpha, Interleukin-1 alpha, mouse IL-1 alpha



Product Information

KE10098 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-1 alpha ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-1 alpha. The assay recognizes mouse IL-1 alpha. An antibody specific for mouse IL-1 alpha has been pre-coated onto the microwells. The mouse IL-1 alpha protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-1 alpha is added to detect the captured mouse IL-1 alpha protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse IL-1 alpha ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatant
Assay type Sandwich
Sensitivity 0.4 pg/mL
Range 4.7-300 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 16175

Recovery

Sample TypeAverageRange
Mouse serum100%76%-124%
Cell culture supernatant85%71%-96%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120135.53.82.8
22030.10.72.4
3207.50.22.8

InterAssay

Samplenmean ( pg/mL)SDCV%
124133.16.24.7
22429.61.34.3
3246.80.56.7

Background Information

Interleukin-1α (IL-1 Alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 Alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. IL-1 Alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 Alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Il 1a, IL1, IL-1, IL1 alpha, IL-1 alpha, IL-1 α, Il1a, IL1alpha, IL-1α, interleukin 1 alpha, Interleukin-1 alpha, mouse IL-1 alpha