Mouse/Rat IGF1 ELISA Kit
Validation Data Gallery
Product Information
KE10032 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse/Rat IGF1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat IGF1. The assay recognizes mouse/rat IGF1. An antibody specific for mouse/rat IGF1 has been pre-coated onto the microwells. The mouse/rat IGF1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse/rat IGF1 is added to detect the captured mouse/rat IGF1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse/Rat IGF1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Mouse serum, Mouse plasma, Rat serum, Rat plasma, Cell lysates, Tissue lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16000 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 105% | 95%-119% |
| Rat serum | 103% | 79%-117% |
| Tissue lysates | 103% | 87%-121% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 550.2 | 16.0 | 2.9 |
| 2 | 20 | 164.8 | 6.7 | 4.1 |
| 3 | 20 | 78.0 | 5.1 | 6.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 501.3 | 38.4 | 7.7 |
| 2 | 24 | 138.5 | 14.1 | 10.2 |
| 3 | 24 | 56.0 | 6.1 | 10.8 |
Background Information
Insulin-like-growth factor 1 (IGF1), a 70 amino-acid peptide hormone is the principal mediator of biochemical effects of growth hormone (GH). IGF1 is an important growth factor in the regulation of cell proliferation and differentiation. IGF1 is largely synthesized in the liver (75%) and, to a lesser extent, in peripheral tissues. IGF1 have been shown to play an essential role in preventing the formation of fatty liver. IGF1 is a potent mitogen and is inhibited by IGF-binding protein-3 (IGFBP3). High serum IGF1 and low IGFBP3 are associated with increased risk of several carcinomas. Mice lacking IGF1 exhibit generalized organ hypoplasia including underdevelopment of the central nervous system and developmental defects in bone, muscle and reproductive systems.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Igf-1, IGF-I, Insulin-like growth factor I, Somatomedin |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Nat Commun Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH) | ||
J Anim Sci Biotechnol Beneficial effects of a decreased meal frequency on nutrient utilization, secretion of luteinizing hormones and ovarian follicular development in gilts. | ||
iScience Exercise-acclimated microbiota improves skeletal muscle metabolism via circulating bile acid deconjugation | ||
Biochem Biophys Res Commun Lactococcus cremoris subsp. cremoris FC-fermented milk activates protein synthesis and increases skeletal muscle mass in middle-aged mice. | ||
Can J Physiol Pharmacol LPS-pretreated MSC-conditioned medium optimized with 10-kDa filter attenuates the injury of H9c2 cardiomyocytes in a model of hypoxia/reoxygenation. | ||
Biol Pharm Bull Juzentaihoto Suppresses Muscle Atrophy and Decreased Motor Function in SAMP8 Mice. |
