Mouse/Rat Flt-3 Ligand/FLT3L ELISA Kit
Cat no : KE10072
Synonyms
Flt3lg, Flt3 ligand, Flt3l, Fms-related tyrosine kinase 3 ligand, Ly72L
Validation Data Gallery
Product Information
KE10072 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse/rat Flt-3 Ligand/FLT3L ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat Flt-3 Ligand/FLT3L. The assay recognizes mouse/rat Flt-3 Ligand/FLT3L. An antibody specific for mouse/rat Flt-3 Ligand/FLT3L has been pre-coated onto the microwells. The mouse/rat Flt-3 Ligand/FLT3L protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse/rat Flt-3 Ligand/FLT3L is added to detect the captured mouse/rat Flt-3 Ligand/FLT3L protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse/Rat Flt-3 Ligand/FLT3L ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Mouse serum, Mouse plasma, Rat serum, Rat plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 3.0 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 14256 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 103% | 95%-113% |
| Rat serum | 102% | 95%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 503.9 | 16.0 | 3.2 |
| 2 | 20 | 117.9 | 2.0 | 1.7 |
| 3 | 20 | 29.4 | 1.7 | 5.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 516.4 | 31.6 | 6.1 |
| 2 | 24 | 121.0 | 5.6 | 4.6 |
| 3 | 24 | 31.6 | 1.8 | 5.8 |
Background Information
Flt-3 Ligand, also known as FLT3L, is a cytokine that is of paramount importance in the proliferation of primitive hematopoietic progenitors. By acting on its receptor Flt-3 (CD135) and synergizing with other cytokines, FLT3L plays a pivotal role in promoting myeloid and lymphoid progenitors proliferation and differentiation. Besides regulating the development of immune cells during steady state conditions, FLT3L levels are raised upon inflammation. For example, increased serum levels of FLT3L has been observed during malaria in mice. Previous studies have shown that mice lacking FLT3L have deficient hematopoiesis function, resulting in reduced numbers of hematopoietic progenitor cells, dendritic cells, and natural killer cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Flt3lg, Flt3 ligand, Flt3l, Fms-related tyrosine kinase 3 ligand, Ly72L |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Signal Transduct Target Ther Oncolytic adenovirus delivery of neoantigens sensitizes low-mutation tumors to anti-PD-1 therapy and prevents metastasis. |
