Mouse/Rat FGF-21 ELISA Kit
Validation Data Gallery
Product Information
KE10042 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse/Rat FGF-21 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat FGF-21. The assay recognizes mouse/rat FGF-21. An antibody specific for mouse/rat FGF-21 has been pre-coated onto the microwells. The mouse/rat FGF-21 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse/rat FGF-21 is added to detect the captured mouse/rat FGF-21 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse/Rat FGF-21 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Mouse serum, Mouse plasma,Rat serum, Rat plasma,Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.4 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 56636 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 87% | 75%-123% |
| Rat serum | 86% | 73%-110% |
| Cell culture supernatants | 87% | 79%-104% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 489.2 | 21.8 | 4.5 |
| 2 | 20 | 119.3 | 4.6 | 3.9 |
| 3 | 20 | 25.5 | 1.1 | 4.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 525.5 | 29.3 | 5.6 |
| 2 | 24 | 120.5 | 4.3 | 3.6 |
| 3 | 24 | 28.5 | 1.6 | 5.5 |
Background Information
Fibroblast growth factor 21 (FGF21) is a peptide hormone that is expressed across several tissues, predominantly in liver and adipocyte tissues, and, to a lower extent, in the pancreas, skeletal muscle, heart, kidneys and testes. FGF21 plays a role in regulating adaptation to various metabolic abnormalities. In the liver, FGF21 plays an important role in the regulation of fatty acid oxidation both in the fasted state and in mice consuming a high-fat, low-carbohydrate ketogenic diet. FGF21 also regulates fatty acid metabolism in mice consuming a diet that promotes hepatic lipotoxicity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | fibroblast growth factor 21, Fgf21, FGF-21 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Sci Adv Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4. | ||
Front Pharmacol Elucidation of the Mechanism of Action of Ginseng Against Acute Lung Injury/Acute Respiratory Distress Syndrome by a Network Pharmacology-Based Strategy. | ||
Biol Pharm Bull Nobiletin and 3'-Demethyl Nobiletin Activate Brown Adipocytes upon β-Adrenergic Stimulation. | ||
Cell Rep The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding. | ||
Pediatr Res FGF21 modulates hippocampal cold-shock proteins and CA2-subregion proteins in neonatal mice with hypoxia-ischemia | ||
Biochem Biophys Res Commun Growth hormone resistance induced by amino acid deprivation in fao cells is independent of FGF21 |
