Mouse Endoglin/CD105 ELISA Kit
Sensitivity
0.01 ng/mL
Range
0.156-10 ng/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
KE10056 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Endoglin/CD105 ELISA kit is to be used to detect and quantify protein levels of endogenous Endoglin/CD105. The assay recognizes mouse Endoglin/CD105. An antibody specific for Endoglin/CD105 has been pre-coated onto the microwells. The Endoglin/CD105 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Endoglin/CD105 is added to detect the captured Endoglin/CD105 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Endoglin/CD105 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 13805 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 96% | 85%-100% |
| Tissue lysates | 89% | 79%-112% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4.67 | 0.20 | 4.4 |
| 2 | 20 | 0.99 | 0.04 | 4.2 |
| 3 | 20 | 0.23 | 0.01 | 4.2 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 5.06 | 0.49 | 9.7 |
| 2 | 24 | 1.09 | 0.08 | 7.5 |
| 3 | 24 | 0.25 | 0.02 | 6.2 |
Background Information
Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. The extracellular domain of membrane-bound Endoglin can be proteolytically cleaved, releasing a soluble form of Endoglin (sCD105). Increased levels of sCD105 are linked to the pathogenesis of severe vascular disease, and also correlate with poor prognosis in patients suffering from various types of cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD105, Edg, endoglin, Eng, S endoglin |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
