Mouse EGF ELISA Kit

Sensitivity

0.7 pg/mL

Range

3.9-250 pg/mL

Reactivity

Mouse

Cat No : KE10093

说明书下载

Synonyms

AI790464, Egf, epidermal growth factor



Product Information

KE10093 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse EGF ELISA kit is to be used to detect and quantify protein levels of endogenous mouse EGF. The assay recognizes mouse EGF. An antibody specific for mouse EGF has been pre-coated onto the microwells. The mouse EGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse EGF is added to detect the captured mouse EGF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse EGF ELISA Kit
Tests 1 X 96 well plate
Sample type Cell culture supernatants, Urine, Tissue homogenates
Assay type Sandwich
Sensitivity 0.7 pg/mL
Range 3.9-250 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 13645

Recovery

Sample TypeAverageRange
Urine109%104%-118%
Cell culture supernatants91%75%-119%
Tissue homogenates90%71%-110%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120130.26.65.1
22032.21.44.5
3208.40.56.0

InterAssay

Samplenmean ( pg/mL)SDCV%
124133.55.94.4
22433.71.75.2
3248.20.89.3

Background Information

Epidermal growth factor (EGF) is a protein that stimulates cell growth and differentiation by binding to EGFR receptor. EGF protein acts a mitogenic factor that plays a role in the growth, proliferation and differentiation of numerous cell types. Salivary EGF can heal oral and gastroesophageal ulcers, inhibiting gastric acid secretion and stimulating mucosal protection. EGF can be found in platelets, urine, saliva, milk, tears, and blood plasma. It can also be found in the submandibular glands and the parotid gland. Testosterone stimulates the production of EGF. By targeting in mice, EGF activates ERBB proteins, forming functional dimers, which provides an explanation for ERBB2/HER2 in human cancer.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms AI790464, Egf, epidermal growth factor