Speedy™ Mouse Chemerin One-Step ELISA Kit
Sensitivity
1.3 pg/mL
Range
7.8-500 pg/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse Chemerin One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.3 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 71660 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 105% | 87%-120% |
| Cell culture supernatant | 104% | 93%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 263.9 | 6.4 | 2.4 |
| 2 | 8 | 58.1 | 2.1 | 3.6 |
| 3 | 8 | 26.6 | 0.6 | 2.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 257.3 | 8.8 | 3.4 |
| 2 | 16 | 57.9 | 2.1 | 3.6 |
| 3 | 16 | 27.1 | 0.9 | 3.3 |
Background Information
Chemerin, also named as TIG2 and RARRES2, is adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Its other ligands include G protein-coupled receptor 1 (GPR1) and chemokine receptor-like 2 (CCRL2). It positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. Chemerin have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Rarres2, AI303516, Retinoic acid receptor responder protein 2 |
