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Recombinant Mouse CTLA-4 protein (His Tag)

种属

Mouse

纯度

>95 %, SDS-PAGE

标签

His Tag

生物活性

EC50: 2-9 μg/mL

Cat no : Eg0632

Print datasheet

Synonyms

CTLA-4, Ctla4, Cd152, Ctla 4, Ly 56

验证数据展示

  • Purity of Recombinant Mouse CTLA-4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CTLA-4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Mouse CTLA-4 (His tag) at 0.5 μg/mL (100 μL/well) can bind B7-1 (Myc tag, His tag, Fc tag) with a linear range of 2-9 ng/mL.

    Immobilized Mouse CTLA-4 (His tag) at 0.5 μg/mL (100 μL/well) can bind B7-1 (Myc tag, His tag, Fc tag) with a linear range of 2-9 ng/mL.

产品信息

纯度 >95 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Immobilized Mouse CTLA-4 (His tag) at 0.5 μg/mL (100 μL/well) can bind Mouse B7-1 (Myc tag, His tag, Fc tag) with a linear range of 2-9 ng/mL.
来源 HEK293-derived Mouse CTLA-4 protein Glu36-Phe162 (Accession# NP_033973) with His tag at the C-terminus.
基因ID 12477
蛋白编号 NP_033973
预测分子量 14.8 kDa
SDS-PAGE 18-23 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).

参考文献:

1. Brunet JF, et al. (1987) Nature. 328(6127):267-70.
2. Harper K, et al. (1991) J Immunol. 147(3):1037-44.
3. McCoy KD, et al. (1999) Immunol Cell Biol. 77(1):1-10.
4. Oaks MK, et al. (2000) Cell Immunol. 201(2):144-53.