Mouse CHI3L1/YKL40 ELISA Kit
Sensitivity
1.7 pg/mL
Range
7.8-500 pg/mL
Reactivity
Mouse
Cat no : KE10090
Synonyms
AW208766, Breast regression protein 39, Brp39, Cartilage glycoprotein 39, CGP 39, Chi3l1, Chil1, chitinase 3 like 1, GP 39, Gp39, YKL40
Validation Data Gallery
Product Information
KE10090 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CHI3L1/YKL40 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CHI3L1/YKL40. The assay recognizes mouse CHI3L1/YKL40. An antibody specific for mouse CHI3L1/YKL40 has been pre-coated onto the microwells. The mouse CHI3L1/YKL40 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CHI3L1/YKL40 is added to detect the captured mouse CHI3L1/YKL40 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CHI3L1/YKL40 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.7 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 12654 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 104% | 84%-124% |
| Cell culture supernatants | 89% | 74%-102% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 185.3 | 6.6 | 3.6 |
| 2 | 20 | 46.5 | 1.8 | 3.9 |
| 3 | 20 | 9.6 | 0.7 | 7.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 187.2 | 8.0 | 4.3 |
| 2 | 24 | 45.6 | 1.5 | 3.4 |
| 3 | 24 | 10.7 | 1.0 | 9.6 |
Background Information
Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39) is a glycoprotein synthesized and secreted by a multitude of cells including macrophages, neutrophils, synoviocytes, chondrocytes, fibroblast-like cells, smooth muscle cells, and tumor cells. It plays a major role in tissue injury, inflammation, tissue repair, and remodeling responses. CHI3L1 has been strongly associated with diseases including asthma, arthritis, sepsis, diabetes, liver fibrosis, and coronary artery disease. CHI3L1 signaling plays a critical role in cancer cell growth, proliferation, invasion, metastasis, angiogenesis, activation of tumor-associated macrophages, and Th2 polarization of CD4+ T cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AW208766, Breast regression protein 39, Brp39, Cartilage glycoprotein 39, CGP 39, Chi3l1, Chil1, chitinase 3 like 1, GP 39, Gp39, YKL40 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
