Mouse CD98/SLC3A2 ELISA Kit
Sensitivity
2.0 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Mouse
Cat no : KE10150
Synonyms
4F2 cell-surface antigen heavy chain, 4F2hc, Amino acid transporter heavy chain SLC3A2, CD98, Mdu1
Validation Data Gallery
Product Information
KE10150 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD98/SLC3A2 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD98/SLC3A2. The assay recognizes mouse CD98/SLC3A2. An antibody specific for mouse CD98/SLC3A2 has been pre-coated onto the microwells. The mouse CD98/SLC3A2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD98/SLC3A2 is added to detect the captured mouse CD98/SLC3A2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD98/SLC3A2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.0 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 17254 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 96% | 85%-102% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,465.7 | 79.0 | 3.2 |
| 2 | 8 | 613.0 | 23.8 | 3.9 |
| 3 | 8 | 317.7 | 8.2 | 2.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,442.3 | 77.5 | 3.2 |
| 2 | 16 | 606.0 | 20.8 | 3.4 |
| 3 | 16 | 312.6 | 10.0 | 3.2 |
Background Information
Solute carrier family 3 member 2 (SLC3A2, also known as CD98 and MDU1) is a 125 kDa type II membrane glycoprotein heterodimer composed of a 40 kDa nonglycosylated light chain and an 85 kDa glycosylated heavy chain. CD98 supports lymphocyte clonal expansion by amplifying integrin signals that enable proliferation and prevent apoptosis. It could function in B and T cell activation, proliferation, or effector functions. Deleting CD98 heavy chain (CD98hc) in B cells leads to complete failure of B cell proliferation, plasma cell formation, and antibody secretion. The CD98 system is independent of the IL-2/IL-2R system in murine T lymphocyte activation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 4F2 cell-surface antigen heavy chain, 4F2hc, Amino acid transporter heavy chain SLC3A2, CD98, Mdu1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
