Recombinant Mouse CD40 Ligand/TNFSF5 protein (His Tag)

种属

Mouse

纯度

>90 %, SDS-PAGE

标签

His Tag

生物活性

未测试

Cat no : Eg0990

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Synonyms

CD154, Cd40l, CD40 ligand, Cd40lg, Tnfsf5



产品信息

纯度 >90 %, SDS-PAGE
内毒素 <1.0 EU/μg protein, LAL method
生物活性
Not tested
来源 HEK293-derived Mouse CD40 Ligand/TNFSF5 protein Gly115-Leu260 (Accession# P27548) with a His tag at the N-terminus.
基因ID 21947
蛋白编号 P27548
预测分子量 17.1 kDa
SDS-PAGE
组分 Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.

参考文献:

1.Mazzei, G J et al. The Journal of biological chemistry vol. 270,13 (1995): 7025-8. 2.Zhang, Bikui et al. Immunology letters vol. 153,1-2 (2013): 58-61. 3.Rizvi, Muhammad et al. Trends in molecular medicine vol. 14,12 (2008): 530-8.