Mouse CD31 ELISA Kit
Sensitivity
0.8 pg/mL
Range
19.5-1250 pg/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
KE10157 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD31 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD31. The assay recognizes mouse CD31. An antibody specific for mouse CD31 has been pre-coated onto the microwells. The mouse CD31 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD31 is added to detect the captured mouse CD31 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD31 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.8 pg/mL |
| Range | 19.5-1250 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 18613 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 92% | 87%-98% |
| Tissue lysate | 99% | 94%-105% |
| Mouse serum | 95% | 84%-118% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 669.0 | 30.3 | 4.5 |
| 2 | 8 | 181.7 | 6.5 | 3.6 |
| 3 | 8 | 49.3 | 3.5 | 7.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 676.3 | 25.0 | 3.7 |
| 2 | 16 | 182.2 | 10.8 | 5.9 |
| 3 | 16 | 51.4 | 3.8 | 7.4 |
Background Information
CD31, also known as platelet endothelial cell adhesion molecule1 (PECAM-1), is a 130 kDa platelet-endothelial cell (EC) adhesion molecule initially identified from ECs and platelets and later from blood leucocytes. CD31 was initially classified as a cell adhesion molecule, later studies suggested that CD31 triggers downstream inhibitory signaling upon trans-homophilic CD31 engagement during cell-cell interaction. CD31 signaling participates in regulating leucocyte detachment, T-cell activation, platelet activation, and angiogenesis, all of which are critical to the pathogenesis of atherosclerosis and abdominal aortic aneurysms (AAAs).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Pecam, PECAM 1, Pecam1, PECAM-1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
