Mouse CD30/TNFRSF8 ELISA Kit
Sensitivity
0.2 pg/mL
Range
19.5-1250 pg/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
KE10144 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD30/TNFRSF8 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD30/TNFRSF8. The assay recognizes mouse CD30/TNFRSF8. An antibody specific for mouse CD30/TNFRSF8 has been pre-coated onto the microwells. The mouse CD30/TNFRSF8 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD30/TNFRSF8 is added to detect the captured mouse CD30/TNFRSF8 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD30/TNFRSF8 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 19.5-1250 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 21941 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 107% | 102%-117% |
| Cell culture supernatant | 109% | 107%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 647.5 | 16.1 | 2.5 |
| 2 | 8 | 149.6 | 2.4 | 1.6 |
| 3 | 8 | 77.5 | 2.1 | 2.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 646.4 | 11.5 | 1.8 |
| 2 | 16 | 150.7 | 2.9 | 1.9 |
| 3 | 16 | 78.1 | 1.9 | 2.5 |
Background Information
CD30, also known as TNFRSF8, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. It plays a crucial role in immune regulation and signaling. CD30 interacts with its ligand, CD30L (CD153), which is expressed on activated T cells, leading to the activation of downstream signaling pathways such as NF-κB, MAPK, and JAK/STAT. These pathways are involved in cell proliferation, survival, and immune response modulation. In mice, soluble CD30 (sCD30) can be detected in serum and other body fluids. Studies in mice have shown that sCD30 can modulate immune responses by competing with membrane-bound CD30 for CD30L binding, thereby reducing the activation of downstream signaling pathways.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD30,CD30L receptor,Lymphocyte activation antigen |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
