Mouse C-Reactive Protein/CRP ELISA Kit
Validation Data Gallery
Product Information
KE10085 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse C-Reactive Protein/CRP ELISA kit is to be used to detect and quantify protein levels of endogenous mouse C-Reactive Protein/CRP. The assay recognizes mouse C-Reactive Protein/CRP. An antibody specific for mouse C-Reactive Protein/CRP has been pre-coated onto the microwells. The mouse C-Reactive Protein/CRP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse C-Reactive Protein/CRP is added to detect the captured mouse C-Reactive Protein/CRP protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse C-Reactive Protein/CRP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma,Tissue lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 12944 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 78%-111% | 91% |
| Tissue lysates | 79%-106% | 90% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 401.7 | 8.9 | 2.2 |
| 2 | 20 | 102.3 | 2.2 | 2.2 |
| 3 | 20 | 24.4 | 0.5 | 2.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 432.6 | 11.8 | 2.7 |
| 2 | 24 | 108.2 | 2.6 | 2.4 |
| 3 | 24 | 27.8 | 0.9 | 3.3 |
Background Information
C-reactive protein (CRP) is an evolutionary highly conserved member of the pentraxin superfamily of proteins. CRP consists of five identical subunits with molecular weight 20-30 kDa arranged as a cyclic pentamer. CRP exists in at least two conformationally distinct forms, native pentameric CRP (pCRP) and modified/monomeric CRP (mCRP). CRP plays important roles in inflammatory processes and host responses to infection including promoting agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its Ca2+-dependent binding to phosphorylcholine (PCh). CRP is mainly expressed by hepatocytes and secreted into plasma.The concentration of CRP will increase significantly in plasma during acute phase response to tissue injury, infection or other inflammatory stimuli.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C reactive protein, C-reactive protein, Crp |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Biomed Pharmacother Resveratrol as a potential therapeutic agent for sarcopenic obesity: Insights from in vivoperiments | ||
Exp Anim Intestinal epithelial cell-specific restoration of Nrf2 gene in whole-body-knockout mice ameliorates acute colitis | ||
J Ethnopharmacol TCM compatibility-driven mechanism of Shenshuaining (SSN) capsules against renal fibrosis: Integrated metabolomic and network analyses. | ||
Nat Immunol The long noncoding RNA lnc13 restrains inflammatory responses to maintain oral tolerance to gluten. |
